Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii
文献类型：期刊论文
| 作者 | Ye, YW ; Wu, QP ; Yao, L ; Dong, XH ; Wu, K ; Zhang, JM |
| 刊名 | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 132期号:40577页码:172-175 |
| 关键词 | Consensus fragment Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR Enterobacter sakazakii Dot hybridization |
| ISSN号 | 0168-1605 |
| 通讯作者 | yeyw04@mails.gucas.ac.cn ; wuqp203@yahoo.com.cn |
| 中文摘要 | Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was employed to generate ERIC-PCR fingerprints of 23 strains of E. sakazakii. A consensus fragment was observed in all 23 strains and was purified, cloned and sequenced from type strain ATCC29544. A comparison of the nucleotide acid with other sequences available in the GenBank revealed 98% of identity with E. sakazakii ATCC BAA-894 and 71%-75% identity with oligopeptidase gene or protease 11 gene of some species from the Enterobacteriaceae family. The consensus fragment from ATCC29544 was used to synthesize probes using DIG-labeling by PCR for dot hybridization. The consensus fragment was found to be highly conserved. Diversity analysis based on the consensus fragment sequencing showed a high heterogeneity between E sakazakii strains and other related strains. In addition, 24 E. sakazakii strains could be distributed into two groups, while E. sakazakii ATCC51329 formed a separate branch. Genetic diversity and potential taxonomic complexity were evident within the E sakazakii strains. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Food Science & Technology; Microbiology |
| 收录类别 | SCI |
| 原文出处 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000267138700013 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5380]  |
| 专题 | 南海海洋研究所_海洋生物 |
| 推荐引用方式 GB/T 7714 | Ye, YW,Wu, QP,Yao, L,et al. Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2009,132(40577):172-175. |
| APA | Ye, YW,Wu, QP,Yao, L,Dong, XH,Wu, K,&Zhang, JM.(2009).Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii.INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,132(40577),172-175. |
| MLA | Ye, YW,et al."Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii".INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 132.40577(2009):172-175. |
入库方式： OAI收割
来源：南海海洋研究所
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