中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
包埋玻璃化法超低温保存植物种质的研究进展

文献类型:期刊论文

作者吴雪梅 ; 汤浩茹
刊名植物学通报
出版日期2006
卷号22期号:2页码:238-245
关键词包埋玻璃化 超低温保存 植物种质保存
其他题名Research Advances in Cryopreservation of Plant Germplasm by Encapsulation-vitrification Method
通讯作者汤浩茹
合作状况国内
中文摘要包埋玻璃化法是在玻璃化法和包埋脱水法基础上发展起来的超低温保存植物种质的新技术。它具有能同时处理大量材料,处理后恢复生长快,对材料的毒害作用较小及成芽率高等优点,已成功地用于辣根、山嵛菜等20余种植物,在植物种质资源的保存上显示出了巨大的应用潜力。本文介绍了包埋玻璃化法产生的背景及其优点,阐述了包埋玻璃化法的基本方法和预培养、包埋、脱水、化冻及恢复培养等过程,比较了该法冻存后的效果和冻存后所形成植株的遗传稳定性,同时指出了进一步研究的重点。
英文摘要Encapsulation-vitrification, a new method for cryopreservation of plant germplasm in recent years, was developed on the basis of vitrification and encapsulation-dehydration. It has many advantages over vitrification and encapsulation-dehydration, because more materials can be treated at the same time, growth is recovered more rapidly after treatment, it is less harmful to the material, and allows for a higher shoot regeneration rate. It has been successfully used to preserve more than 20 species, such as pear, horseradish and wasabi, and shows great applica- tion potential in the preservation of plant germplasm resources. This paper introduces the devel- opment and advantages of encapsulation-vitrification and the main methods and procedures such as preculture, encapsulation, dehydration, thawing and recovery of culture. The effects of freezing protection and the genetic stability of plant materials preserved with encapsulation- vitrification are discussed as well. Further research on encapsulation-vitrification of plant tissue is also suggested.
分类号Q943
资助信息教育部优秀青年教师计划;全国优秀博士学位论文作者博士专项基金(200253)项目
语种中文
公开日期2010-11-12
源URL[http://ir.imde.ac.cn/handle/131551/2535]  
专题成都山地灾害与环境研究所_成都山地所知识仓储(2009年以前)
推荐引用方式
GB/T 7714
吴雪梅,汤浩茹. 包埋玻璃化法超低温保存植物种质的研究进展[J]. 植物学通报,2006,22(2):238-245.
APA 吴雪梅,&汤浩茹.(2006).包埋玻璃化法超低温保存植物种质的研究进展.植物学通报,22(2),238-245.
MLA 吴雪梅,et al."包埋玻璃化法超低温保存植物种质的研究进展".植物学通报 22.2(2006):238-245.

入库方式: OAI收割

来源:成都山地灾害与环境研究所

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