茶树菇原生质体的分离和再生以及单核菌株的筛选
文献类型：期刊论文
| 作者 | 邱敦莲
|
| 刊名 | 西南大学学报(自然科学版)
 |
| 出版日期 | 2008-12 |
| 卷号 | 30期号:12页码:116-120 |
| 关键词 | 茶树菇+ 原生质体+ 分离+ 再生+单核菌株+ 筛选 |
| 通讯作者 | 杨志荣 |
| 合作状况 | 国内 |
| 中文摘要 | 研究了利用珍稀食用茵茶树菇双核茵丝进行原生质体分离和再生,以及原生质体单核菌株的筛选过程.利用培养4～6 d的茶树菇双核茵丝,以0.6 mol/mL MgSO4·7H2O作稳定荆,在溶壁酶浓度为4 0 mg/mL,温度30～32℃的摇床振荡(70 r/min)条件下进行酶解4 h,制备原生质体,然后,将原生质体在下层高渗固体培养基和上层高渗半固体培养基上再生,从该再生茵落中筛选到茶树菇单核菌株1株. |
| 英文摘要 | The preparation of monokaryon protoplast s and the screening of protoplast-derived monokaryon st rains are important part s in mushroom research and breeding. As monokaryon protoplasts are derived directly f rom mycelia wit hout the process of chromosome meiosis instead of f rom spores , t he genetic characteristics of the donor parent can be conserved. Agrocye chaxingu Huang is a rare and precious edible mushroom species. This paper report s the protocol of isolation and regeneration of A1 chaxingu protoplasts, and the selection of protoplast-derived monokaryon st rains. One monokaryon st rain in A1 chaxingu was obtained by the following steps. First, the mycelia precultured for 4 - 6 days were hydrolyzed with lywallzyme at a concentration of 40 mg/ mL and with 0.16 mol/ mL MgSO4 ·7H2O as the osmotic stabilizer on the shaker at 30 - 32 ℃and 70 r/ min for 4 hours. Then, the protoplasts were inoculated on a solid medium with high osmotic pressure and covered by a semi-solid medium wit h high osmotic pressure to regenerate, and colonies which grew slower and were smaller were picked one by one and checked under a microscope to observe t he mycelia morphology and clamp-like structures. Consequently, one monokaryon strain was screened from the regenerated colonies. |
| 学科主题 | 微生物学 |
| 收录类别 | CSCD |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3590670 |
| 公开日期 | 2012-06-26 |
| 源URL | [http://192.168.143.20:8080/handle/131551/4057]  |
| 专题 | 成都山地灾害与环境研究所_管理与支撑系统 科技信息与传播中心 |
| 推荐引用方式 GB/T 7714 | 邱敦莲. 茶树菇原生质体的分离和再生以及单核菌株的筛选[J]. 西南大学学报(自然科学版),2008,30(12):116-120. |
| APA | 邱敦莲.(2008).茶树菇原生质体的分离和再生以及单核菌株的筛选. 西南大学学报(自然科学版),30(12),116-120. |
| MLA | 邱敦莲."茶树菇原生质体的分离和再生以及单核菌株的筛选". 西南大学学报(自然科学版) 30.12(2008):116-120. |
入库方式： OAI收割
来源：成都山地灾害与环境研究所
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