Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum
文献类型：期刊论文
| 作者 | Chen, Ning1; Du, Jun1; Liu, Hui2; Xu, Qingyang1 |
| 刊名 | ANNALS OF MICROBIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 59期号:2页码:317-322 |
| 关键词 | Corynebacterium glutamicum L-glutamate metabolic pathway analysis optimization |
| ISSN号 | 1590-4261 |
| 通讯作者 | Chen, N |
| 英文摘要 | In this study we applied elementary mode analysis and metabolic flux analysis to L-glutamate biosynthesis by Corynebacterium glutamicum GDK-9. The theoretically optimal flux distribution with the maximal L-glutamate yield and the actual flux distribution of L-glutamate production were determined, which suggested that phosphoenol-pyruvate (PEP), 2-oxoglutarate (2-OXO) and iso-citric acid (ICI) were the key nodes of L-glutamate biosynthesis. It was also indicated that the carbon-fixation reaction from CO(2) had great influence on the flux distribution of the key nodes. Besides, the biosynthesis of lactate could greatly decrease the L-glutamate yield. Therefore we further developed a rational dissolved oxygen control strategy to improve the L-glutamate production by strengthening the carbon-fixation reaction from CO2 and reducing the lactate production. Under the optimized fermentation conditions, a final L-glutamate concentration of 141 g/L was obtained and the carbon yield of L-glutamate on glucose was 81.7%. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | L-THREONINE PRODUCTION ; ESCHERICHIA-COLI ; CULTURE-CONDITIONS ; NETWORK ANALYSIS ; PATHWAY ANALYSIS ; OPTIMIZATION ; IMPROVEMENT ; BATCH ; OVERPRODUCTION ; FERMENTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267606200018 |
| 公开日期 | 2013-12-11 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6767]  |
| 专题 | 过程工程研究所_多相复杂系统国家重点实验室 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ning,Du, Jun,Liu, Hui,et al. Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum[J]. ANNALS OF MICROBIOLOGY,2009,59(2):317-322. |
| APA | Chen, Ning,Du, Jun,Liu, Hui,&Xu, Qingyang.(2009).Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum.ANNALS OF MICROBIOLOGY,59(2),317-322. |
| MLA | Chen, Ning,et al."Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis by Corynebacterium glutamicum".ANNALS OF MICROBIOLOGY 59.2(2009):317-322. |
入库方式： OAI收割
来源：过程工程研究所
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