Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin
文献类型：期刊论文
| 作者 | Zhang, Guifeng1; Liu, Tao1; Wang, Qian2; Chen, Li3; Lei, Jiandu1; Luo, Jian1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | FOOD HYDROCOLLOIDS
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 23期号:7页码:2001-2007 |
| ISSN号 | 0268-005X |
| 关键词 | Bovine gelatin Porcine gelatin Tryptic digestion Marker peptide HPLC/MS |
| 通讯作者 | Su, ZG |
| 英文摘要 | Gelatin is a mixture of polypeptides obtained by hydrolysis of collagen primarily from bovine and porcine skin and bones. The similarity between different gelatins makes it difficult to trace their species origin. In this work, a new method for differentiation between bovine and porcine gelatin was developed based on detection and identification of marker peptides in digested gelatins. Sequence alignment analysis indicates that bovine and porcine Type I collagen contain differential sequences. The gelatins were digested by trypsin, and the resulting peptides were analyzed by high performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). The marker peptides specific for bovine and porcine were successfully detected in the digested bovine and porcine gelatin, respectively. Comparative analysis indicated that more marker peptides could be detected in gelatin with a higher mean molecular weight. It was found that proline hydroxylation was a key factor affecting the peptide identification. For peptides such as GPPGSAGSPGK and GPPGSAGAPGK detected in digested bovine and porcine gelatin, respectively, the sequence should be verified manually since the mass shift caused by proline hydroxylation can be confused with the mass difference between Set and Ala residues. The results indicate that detection of marker peptides in the digested gelatin sample using HPLC-MS/MS is an effective method to differentiate between bovine and porcine gelatin. (C) 2009 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 类目[WOS] | Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Chemistry ; Food Science & Technology |
| 关键词[WOS] | PROTEIN IDENTIFICATION ; COLLAGEN ; HYDROXYLATION ; STABILIZATION ; PROTEOMICS ; PROLINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267478400047 |
| 公开日期 | 2013-11-29 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6572]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 1000029, Peoples R China 3.Bioinformat Inst, Singapore 138671, Singapore |
| 推荐引用方式 GB/T 7714 | Zhang, Guifeng,Liu, Tao,Wang, Qian,et al. Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin[J]. FOOD HYDROCOLLOIDS,2009,23(7):2001-2007. |
| APA | Zhang, Guifeng.,Liu, Tao.,Wang, Qian.,Chen, Li.,Lei, Jiandu.,...&Su, Zhiguo.(2009).Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin.FOOD HYDROCOLLOIDS,23(7),2001-2007. |
| MLA | Zhang, Guifeng,et al."Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin".FOOD HYDROCOLLOIDS 23.7(2009):2001-2007. |
入库方式： OAI收割
来源：过程工程研究所
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