A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography
文献类型：期刊论文
| 作者 | Qu, Jian-Bo1,2; Wan, Xing-Zhong3; Zhai, Yan-Qin1,2; Zhou, Wei-Qing1; Su, Zhi-Guo1; Ma, Guang-Hui1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2009-09-11 |
| 卷号 | 1216期号:37页码:6511-6516 |
| 关键词 | Gigaporous Polystyrene microspheres Anion exchanger Protein chromatography Column efficiency Dynamic binding capacity |
| ISSN号 | 0021-9673 |
| 通讯作者 | Ma, GH |
| 英文摘要 | Using agarose coated gigaporous polystyrene microspheres as a base support, a novel anion exchanger (DEAE-AP) has been developed after functionalization with diethylaminoethyl chloride. The gigaporous structure, static adsorption behavior, and chromatographic properties of DEAE-AP medium were characterized and compared with those of commercially available resin DEAE Sepharose Fast Flow (DEAE-FF). The results implied that there existed some through pores in DEAE-AP microspheres, which effectively reduced resistance to stagnant mobile phase mass transfer by inducing convective flow of mobile phase in the gigapores of medium. As a consequence, the column packed with DEAE-AP exhibited low column backpressure, high column efficiency, high dynamic binding capacity and high protein resolution at high flow velocity up to 2600cm/h. In conclusion, all the results suggested that the gigaporous absorbent is promising for high-speed protein chromatography. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | PERFUSION CHROMATOGRAPHY ; SEPARATION ; PURIFICATION ; SUPPORTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000269631500007 |
| 公开日期 | 2013-11-29 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6583]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Beijing Baiao Pharmaceut Co Ltd, Beijing 102200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Jian-Bo,Wan, Xing-Zhong,Zhai, Yan-Qin,et al. A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(37):6511-6516. |
| APA | Qu, Jian-Bo,Wan, Xing-Zhong,Zhai, Yan-Qin,Zhou, Wei-Qing,Su, Zhi-Guo,&Ma, Guang-Hui.(2009).A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography.JOURNAL OF CHROMATOGRAPHY A,1216(37),6511-6516. |
| MLA | Qu, Jian-Bo,et al."A novel stationary phase derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed protein chromatography".JOURNAL OF CHROMATOGRAPHY A 1216.37(2009):6511-6516. |
入库方式： OAI收割
来源：过程工程研究所
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