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Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development

文献类型：期刊论文

作者Tang, Ya-Jie1,2,3; Li, Yan2; Zhong, Jian-Jiang4
刊名BIOPROCESS AND BIOSYSTEMS ENGINEERING
出版日期2009-08-01
卷号32期号:5页码:663-671
关键词Biotransformation process development Podophyllotoxin Podophyllic acid Picropodophyllotoxin Pseudomonas aeruginosa
ISSN号1615-7591
通讯作者Tang, YJ
英文摘要A novel biotransformation process of podophyllotoxin (1) to produce picropodophyllotoxin (2) and podophyllic acid (3) was developed in this work. Eight bacteria which could modify the structure of podophyllotoxin were screened out from the tested fourteen bacteria. The highest conversion of podophyllotoxin (i.e., 70.2 +/- A 8.0%) was obtained when Pseudomonas aeruginosa CCTCC AB93066 was used as biocatalyst, so P. aeruginosa was selected as a typical biocatalyst in the following study. Product (2) and (3) were separated through D312 macroporous resin and sephadex LH-20 gel column chromatograph. On the basis of (1)H NMR, (13)C NMR, ESI-MS and Elemental Analysis, product (2) and (3) were identified as picropodophyllotoxin (2) and podophyllic acid (3), respectively. This suggested the site-specific isomerization and hydrolization of podophyllotoxin occurred during its biotransformation process by P. aeruginosa. For the first time, podophyllotoxin was biotransformed into its hydrolytic derivate (i.e., podophyllic acid).
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]SPIN-LABELED DERIVATIVES ; CELL-SUSPENSION CULTURES ; CONGENERS ; LIGNANS ; DEOXYPODOPHYLLOTOXIN ; BIOCONVERSION ; DISCOVERY ; ETOPOSIDE ; ANTITUMOR ; ANALOGS
收录类别SCI
语种英语
WOS记录号WOS:000268294600013
公开日期2013-12-05
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/6608]  
专题过程工程研究所_生化工程国家重点实验室
作者单位1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Hubei Univ Technol, Coll Bioengn, Key Lab Fermentat Engn, Minist Educ,Hubei Prov Key Lab Ind Microbiol, Wuhan 430068, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
4.Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Key Lab Microbial Metab, Minist Educ, Shanghai 200240, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Ya-Jie,Li, Yan,Zhong, Jian-Jiang. Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2009,32(5):663-671.
APA Tang, Ya-Jie,Li, Yan,&Zhong, Jian-Jiang.(2009).Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development.BIOPROCESS AND BIOSYSTEMS ENGINEERING,32(5),663-671.
MLA Tang, Ya-Jie,et al."Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066. Part I: Process development".BIOPROCESS AND BIOSYSTEMS ENGINEERING 32.5(2009):663-671.

入库方式： OAI收割

来源：过程工程研究所

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