A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization
文献类型：期刊论文
| 作者 | Suo, Xiaoyan1,2; Lu, Xiuling1; Hu, Tao1; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 31期号:8页码:1191-1196 |
| 关键词 | Human serum albumin PEGylation solid-phase Staphylokinase |
| ISSN号 | 0141-5492 |
| 通讯作者 | Hu, T |
| 英文摘要 | A solid-phase adsorption method was developed to circumvent the disadvantage of the conventional liquid-phase PEGylation, i.e. the heterogeneity of the PEGylated products. The model proteins, human serum albumin (HSA) and staphylokinase (SAK), were adsorbed on the ion exchange chromatography media, followed by PEGylation with succinimidyl carbonate (SC)-mPEG5K and salt elution. Since PEGylation with SC-PEG5K alters the positive charge of the proteins, Q-Sepharose Big Beads and DEAE Sepharose Fast Flow were used for adsorption of HSA and SAK, respectively. Size exclusion chromatography and SDS-PAGE studies demonstrated that solid-phase PEGylation of proteins generate monoPEGylated proteins with the yield of 35-47%. Circular dichroism and intrinsic fluorescence studies showed that solid-phase PEGylation led to little conformational change of the proteins. Solid-phase PEGylation resulted in 35% loss in the biological activity of SAK, which is lower than the liquid-phase PEGylation (70%). |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | HEMOGLOBIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268002100010 |
| 公开日期 | 2013-12-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6609]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suo, Xiaoyan,Lu, Xiuling,Hu, Tao,et al. A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization[J]. BIOTECHNOLOGY LETTERS,2009,31(8):1191-1196. |
| APA | Suo, Xiaoyan,Lu, Xiuling,Hu, Tao,Ma, Guanghui,&Su, Zhiguo.(2009).A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization.BIOTECHNOLOGY LETTERS,31(8),1191-1196. |
| MLA | Suo, Xiaoyan,et al."A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization".BIOTECHNOLOGY LETTERS 31.8(2009):1191-1196. |
入库方式： OAI收割
来源：过程工程研究所
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