Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers
文献类型：期刊论文
| 作者 | Yuan, X. F.; Dai, Z. H.; Wang, X. D.; Zhao, B. |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 31期号:8页码:1279-1287 |
| 关键词 | Genetic stability ISSR Plant tissue culture RAPD Saussurea involucrata |
| ISSN号 | 0141-5492 |
| 通讯作者 | Yuan, XF |
| 英文摘要 | To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The genetic stability/variation in tissue-cultured products, including three calli, three adventitious shoots, regenerated plantlets and 2 year-old regenerated plantlets cultivated in the planting base in Tianshan Mountain, were assessed compared with 1 year-old and 2 year-old seedlings cultivated in the same planting base using aseptic seedlings as reference. Apparent genetic variation was detected in the 11 type of plant materials. The percentages of polymorphic bands in the RAPD and ISSR analysis were, respectively, 35% and 33%. Cluster analysis indicated that the genetic similarity values calculated on the basis of RAPD and ISSR data among the 11 type of plant materials were respectively ranged from 0.823 to 0.995 with a mean of 0.878 and 0.825 to 0.974 with a mean of 0.885, which classified the samples into three groups. The similarity coefficient also revealed that differences among three calli were not remarkable by both RAPD and ISSR analysis, and only chemical components and growth properties needed consideration in the screening of callus used for the next redifferentiation studies. But there are remarkable differences among three adventitious shoots analyzed by ISSR markers. Therefore, RAPD and ISSR markers are efficient tools in genetic variation assessment and quality control in plant tissue culture process. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SOMACLONAL VARIATION ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268002100025 |
| 公开日期 | 2013-12-05 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6610]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, X. F.,Dai, Z. H.,Wang, X. D.,et al. Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers[J]. BIOTECHNOLOGY LETTERS,2009,31(8):1279-1287. |
| APA | Yuan, X. F.,Dai, Z. H.,Wang, X. D.,&Zhao, B..(2009).Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers.BIOTECHNOLOGY LETTERS,31(8),1279-1287. |
| MLA | Yuan, X. F.,et al."Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers".BIOTECHNOLOGY LETTERS 31.8(2009):1279-1287. |
入库方式： OAI收割
来源：过程工程研究所
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