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Magnetic nanoparticles supported ionic liquids for lipase immobilization: Enzyme activity in catalyzing esterification
文献类型：期刊论文
| 作者 | Jiang, Yangyang; Guo, Chen; Xia, Hansong; Mahmood, Iram; Liu, Chunzhao; Liu, Huizhou |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 58期号:1-4页码:103-109 |
| 关键词 | Esterification Ionic liquids Lipase immobilization Magnetic nanoparticle |
| ISSN号 | 1381-1177 |
| 通讯作者 | Guo, C |
| 英文摘要 | Candida rugosa lipase was immobilized on magnetic nanoparticles supported ionic liquids having different cation chain length (C(1), C(4) and C(8)) and anions (Cl(-), BF(4)(-) and PF(6)(-)). Magnetic nanoparticles Supported ionic liquids were obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. The particles are superparamagnetic with diameter of about 55 nm. Large amount of lipase (63.89 mg/(100 mg carrier)) was loaded on the support through ionic adsorption. Activity of the immobilized lipase was examined by the catalysis of esterification between oleic acid and butanol. The activity of bound lipase was 118.3% compared to that of the native lipase. Immobilized lipase maintained 60% of its initial activity even when the temperature was LIP to 80 degrees C. In addition, immobilized lipase retained 60% of its initial activity after 8 repeated batches reaction, while no activity was detected after 6 cycles for the free enzyme. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | CANDIDA-RUGOSA ; SILICA ; BIOCATALYST ; PERFORMANCE ; HYDROLYSIS ; ADSORPTION ; PARTICLES ; STABILITY ; CATALYSIS ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265584200017 |
| 公开日期 | 2013-12-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6663]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yangyang,Guo, Chen,Xia, Hansong,et al. Magnetic nanoparticles supported ionic liquids for lipase immobilization: Enzyme activity in catalyzing esterification[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2009,58(1-4):103-109. |
| APA | Jiang, Yangyang,Guo, Chen,Xia, Hansong,Mahmood, Iram,Liu, Chunzhao,&Liu, Huizhou.(2009).Magnetic nanoparticles supported ionic liquids for lipase immobilization: Enzyme activity in catalyzing esterification.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,58(1-4),103-109. |
| MLA | Jiang, Yangyang,et al."Magnetic nanoparticles supported ionic liquids for lipase immobilization: Enzyme activity in catalyzing esterification".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 58.1-4(2009):103-109. |
入库方式： OAI收割
来源：过程工程研究所
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