Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption
文献类型：期刊论文
| 作者 | Xu, J.; Chen, H. |
| 刊名 | APPLIED BIOCHEMISTRY AND MICROBIOLOGY
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 45期号:3页码:309-312 |
| 关键词 | LIGNOCELLULOSIC BIOMASS ENZYMATIC-HYDROLYSIS NEWSPAPER |
| ISSN号 | 0003-6838 |
| 通讯作者 | Xu, J |
| 英文摘要 | Through determination of cellulase in terms of protein content in the hydrolyzate after hydrolysis, it was found that 50.15-67.21% of the originally added protein was still remained in the hydrolyzate. Tannin flocculation followed by PEG desorption was coupled to recover this portion of cellulase. About 94.99% of the protein in the hydrolyzate was precipitated into Tannin-cellulase compound under tannin concentration of 5% (w/v) with pH 5.0 and NaCl concentration of 1.0%. In order to desorb cellulase from Tannin-cellulase compound, PEG 6000 was employed as a desorbent. The recovered enzyme activity reached 133.25% when the PEG concentration was 1.5% (w/v). This, to some extent, indicated that PEG was also an effective activator of cellulase. The article is published in the original. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | LIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; NEWSPAPER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265939300012 |
| 公开日期 | 2013-12-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6674]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | CAS, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, J.,Chen, H.. Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption[J]. APPLIED BIOCHEMISTRY AND MICROBIOLOGY,2009,45(3):309-312. |
| APA | Xu, J.,&Chen, H..(2009).Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption.APPLIED BIOCHEMISTRY AND MICROBIOLOGY,45(3),309-312. |
| MLA | Xu, J.,et al."Coupling recovery strategy of cellulase in hydrolyzate after hydrolysis with Tannin flocculation and PEG desorption".APPLIED BIOCHEMISTRY AND MICROBIOLOGY 45.3(2009):309-312. |
入库方式： OAI收割
来源：过程工程研究所
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