Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.
文献类型：期刊论文
| 作者 | Zhang, Dalei1,2,3; Li, Xiunan1; Hao, Dongxia1; Li, Guisheng4; Xu, Benming4; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | SEPARATION AND PURIFICATION TECHNOLOGY
 |
| 出版日期 | 2009-04-20 |
| 卷号 | 66期号:2页码:329-339 |
| 关键词 | Polygonum cuspidatum Sieb. et Zucc. Polyclatin Resveratrol Anthraglycoside B Purification |
| ISSN号 | 1383-5866 |
| 通讯作者 | Su, ZG |
| 英文摘要 | For comprehensive utilization of Polygonum cuspidatum Sieb. et Zucc., a systematic, environmental friendly preparative process was developed for purification of polydatin, resveratrol and anthraglycoside B simultaneously from the herb. The process was simple, consisting of macroporous resin adsorption and reversed-phase liquid chromatography. Macroporous resin adsorption separated the solvent extract into fraction I containing polydatin and fraction II containing resveratrol as well as anthraglycoside B. The fractions I and II were further purified by reversed-phase chromatography to get the fractions corresponding to polydatin, resveratrol and anthraglycoside B, respectively. Two polydivinylbenzene microspheres (PDVB-SPG and PDVB-Swelling) were prepared by SPG membrane emulsification and by two-step activated swelling polymerization, respectively. These new media were compared with a commercial C(18) bonded silica medium in the reversed-phase liquid chromatography step. The PDVB-SPG column demonstrated a better separation compared with C(18) and PDVB-Swelling column. The pH value of the mobile phase showed little effect on the separation of polydatin and resveratrol, and large effect on the separation of anthraglycoside B for both HPLC analysis and preparative reversed-phase liquid chromatography. After the chromatographic process and a further crystallization, polydatin, resveratrol and anthraglycoside B were purified from 7.5%, 1.3% and 2.4% to 98.8%, 98.2% and 98.6%, respectively, with total recoveries of 81.3%,77.4% and 80.2%. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; SEPARATION ; COPOLYMERS ; RETENTION ; PICEID ; ACIDS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265515700017 |
| 公开日期 | 2013-12-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6682]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shandong Engn Res Ctr Nat Drugs, Yantai 264003, Shandong, Peoples R China 4.Yantai Univ, Sch Pharm, Yantai 264005, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dalei,Li, Xiunan,Hao, Dongxia,et al. Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2009,66(2):329-339. |
| APA | Zhang, Dalei.,Li, Xiunan.,Hao, Dongxia.,Li, Guisheng.,Xu, Benming.,...&Su, Zhiguo.(2009).Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc..SEPARATION AND PURIFICATION TECHNOLOGY,66(2),329-339. |
| MLA | Zhang, Dalei,et al."Systematic purification of polydatin, resveratrol and anthraglycoside B from Polygonum cuspidatum Sieb. et Zucc.".SEPARATION AND PURIFICATION TECHNOLOGY 66.2(2009):329-339. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。