Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization
文献类型：期刊论文
| 作者 | Li, Yan1; Li, Yuan-Yuan1; Mi, Zhi-Yuan1; Li, Dong-Sheng1; Tang, Ya-Jie1,2,3 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 100期号:7页码:2271-2277 |
| 关键词 | Biotransformation process optimization Podophyllotoxin Podophyllic acid Fed-batch biotransformation Pseudomonas aeruginosa |
| ISSN号 | 0960-8524 |
| 通讯作者 | Tang, YJ |
| 英文摘要 | This work optimized the novel biotransformation process of podophyllotoxin to produce podophyllic acid by Pseudomonas aeruginosa CCTCC AB93066. Firstly, the biotransformation process was significantly affected by medium composition. 5 g/l of yeast extract and 5 g/l of peptone were favorable for podophyllic acid production (i.e. 25.3 +/- 3.7 mg/l), while not beneficial for the cell growth of P. aeruginosa. This indicated that the accumulation of podophyllic acid was not corresponded well to the cell growth of P. aeruginosa. 0 g/l of sucrose was beneficial for podophyllic acid production (i.e. 34.3 +/- 3.9 mg/l), which led to high podophyllotoxin conversion (i.e. 98.2 +/- 0.1%). 1 g/l of NaCl was the best for podophyllic acid production (i.e. 47.6 +/- 4.0 mg/l). Secondly, the production of podophyllic acid was significantly enhanced by fed-batch biotransformation. When each 100 mg/l of podophyllotoxin was added to the biotransformation system after 4, 10 and 25 h of culture, respectively, podophyllic acid concentration reached 99.9 +/- 12.3 mg/l, enhanced by 284% comparing to one-time addition (i.e. 26.0 +/- 2.1 mg/l). The fundamental information obtained in this study provides a simple and efficient way to produce podophyllic acid. (C) 2008 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | SPIN-LABELED DERIVATIVES ; TOLUENE HYDROXYLATION ; PUTIDA UV4 ; DISCOVERY ; SUBSTRATE ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263018300027 |
| 公开日期 | 2013-12-09 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6689]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Hubei Univ Technol, Coll Bioengn, Hubei Prov Key Lab Ind Microbiol, Wuhan 430068, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yan,Li, Yuan-Yuan,Mi, Zhi-Yuan,et al. Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization[J]. BIORESOURCE TECHNOLOGY,2009,100(7):2271-2277. |
| APA | Li, Yan,Li, Yuan-Yuan,Mi, Zhi-Yuan,Li, Dong-Sheng,&Tang, Ya-Jie.(2009).Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization.BIORESOURCE TECHNOLOGY,100(7),2271-2277. |
| MLA | Li, Yan,et al."Novel biotransformation process of podophyllotoxin to produce podophyllic acid and picropodophyllotoxin by Pseudomonas aeruginosa CCTCC AB93066, Part II: Process optimization".BIORESOURCE TECHNOLOGY 100.7(2009):2271-2277. |
入库方式： OAI收割
来源：过程工程研究所
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