Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using beta-cyclodextrin as the eluent
文献类型：期刊论文
| 作者 | Li, Jing Jing1; Wang, Ai-Qing1; Janson, Jan-Christer2; Ballagi, Andras2; Chen, Jing1; Liu, Yong-Dong1; Ma, Guang-Hui1; Su, Zhi-Guo1 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 44期号:3页码:277-282 |
| 关键词 | Immobilized Triton X-100 Sepharose High Performance Refolding Green Fluorescent Protein Hydrophobic interaction chromatography Artificial chaperone beta-Cyclodextrin |
| ISSN号 | 1359-5113 |
| 通讯作者 | Su, ZG |
| 英文摘要 | A new protein refolding technique based on the use of the non-charged detergent Triton X-100 immobilized to the cross-linked agarose gel Sepharose High Performance has been developed. The new solid phase was used in combination with soluble beta-cyclodextrin (beta-CD) to refold recombinant Green Fluorescent Protein fused to Tobacco Etch Virus protease (GFPTEVP) expressed as inclusion bodies in E. coli. Previous attempts to refold recombinant GFPTEVP by dilution had failed. In the new procedure a column packed with Triton X-100-coupled Sepharose High Performance was used to capture unfolded GFPTEVP followed by elution using an increasing beta-CD concentration gradient. The yield of properly refolded GFPTEVP was 46% at a protein concentration of 380 mu g/ml. In contrast, dilution refolding of GFPTEVP at 200 mu g/ml refolding buffer resulted in only 4.7% of native protein. (C) 2008 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | HYDROPHOBIC INTERACTION CHROMATOGRAPHY ; ARTIFICIAL CHAPERONE ; ESCHERICHIA-COLI ; INCLUSION-BODIES ; RENATURATION ; DETERGENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264148400004 |
| 公开日期 | 2013-12-10 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6715]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Uppsala Univ, Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden |
| 推荐引用方式 GB/T 7714 | Li, Jing Jing,Wang, Ai-Qing,Janson, Jan-Christer,et al. Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using beta-cyclodextrin as the eluent[J]. PROCESS BIOCHEMISTRY,2009,44(3):277-282. |
| APA | Li, Jing Jing.,Wang, Ai-Qing.,Janson, Jan-Christer.,Ballagi, Andras.,Chen, Jing.,...&Su, Zhi-Guo.(2009).Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using beta-cyclodextrin as the eluent.PROCESS BIOCHEMISTRY,44(3),277-282. |
| MLA | Li, Jing Jing,et al."Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using beta-cyclodextrin as the eluent".PROCESS BIOCHEMISTRY 44.3(2009):277-282. |
入库方式： OAI收割
来源：过程工程研究所
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