Solid Phase Pegylation of Hemoglobin
文献类型：期刊论文
| 作者 | Suo, Xiaoyan; Zheng, Chunyang; Yu, Pengzhan; Lu, Xiuling; Ma, Guanghui; Su, Zhiguo |
| 刊名 | ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 37期号:4页码:147-155 |
| 关键词 | solid phase polyethylene glycol hemoglobin red blood cell substitute |
| ISSN号 | 1073-1199 |
| 通讯作者 | Su, ZG |
| 英文摘要 | A solid phase conjugation process was developed for attachment of polyethylene glycol to hemoglobin molecule. Bovine hemoglobin was loaded onto an ion exchange chromatography column and adsorbed by the solid medium. Succinimidyl carbonate mPEG was introduced in the mobile phase after the adsorption. Pegylation took place between the hemoglobin on the solid phase, and the pegylation reagent in the liquid phase. A further elution was carried out to separate the pegylated and the unpegylated protein. Analysis by HPSEC, SDS-PAGE, and MALLS demonstrated that the fractions eluted from the solid phase contained well-defined components. Pegylated hemoglobin with one PEG chain was obtained with the yield of 75%, in comparison to the yield of 30% in the liquid phase pegylation. The P50 values of the mono-pegylated hemoglobin, prepared with SC-mPEG 5kDa, 10kDa and 20kDa, were 19.97, 20.23 and 20.54 mmHg, which were much closer to the value of red blood cells than that of pegylated hemoglobin prepared with the conventional method. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| 关键词[WOS] | BOVINE SERUM-ALBUMIN ; POLYETHYLENE-GLYCOL ; COVALENT ATTACHMENT ; BLOOD ; PROTEINS ; SURFACE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267835500001 |
| 公开日期 | 2013-12-11 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6768]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suo, Xiaoyan,Zheng, Chunyang,Yu, Pengzhan,et al. Solid Phase Pegylation of Hemoglobin[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2009,37(4):147-155. |
| APA | Suo, Xiaoyan,Zheng, Chunyang,Yu, Pengzhan,Lu, Xiuling,Ma, Guanghui,&Su, Zhiguo.(2009).Solid Phase Pegylation of Hemoglobin.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,37(4),147-155. |
| MLA | Suo, Xiaoyan,et al."Solid Phase Pegylation of Hemoglobin".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 37.4(2009):147-155. |
入库方式： OAI收割
来源：过程工程研究所
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