Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration
文献类型：期刊论文
| 作者 | Liu, Wenfang3,4; Zhang, Songping3; Wang, Ping1,2 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 139期号:1页码:102-107 |
| 关键词 | Nanoparticles Enzyme immobilization Multi-enzyme biotransformation Cofactor regeneration Bioprocessing Biosynthesis |
| ISSN号 | 0168-1656 |
| 通讯作者 | Wang, P |
| 英文摘要 | Although there have been a long history of studying and using immobilized enzymes. little has been reported regarding the nature of immobilized cofactors. Herein we report that cofactor NAD(H) covalently attached to silica nanoparticles successfully coordinated with particle-immobilized enzymes and enabled multistep biotransformations. Specifically, silica nanoparticle-attached glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH) and NAD(H) were prepared and applied to catalyze the coupled reactions for production of alpha-ketoglutarate and lactate with the cofactor regenerated within the reaction cycle. It appeared that particle-particle collision driven by Brownian motion of the nanoparticles provided effective interactions among the catalytic components, and thus realized a dynamic shuttling of the particle-supported cofactor between the two enzymes to keep the reaction cycles continuing. Total turnover numbers (TTNs) as high as 20,000 h(-1) were observed for the cofactor. It appeared to us that the use of particle-attached cofactor promises a new biochemical processing strategy for cofactor-dependent biotransformations. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CHARGED MEMBRANE BIOREACTOR ; ALCOHOL-DEHYDROGENASE ; NADH REGENERATION ; NAD(P)-DEPENDENT OXIDOREDUCTASES ; SORBITOL PRODUCTION ; IMMOBILIZED NAD ; ENZYME REACTOR ; COENZYME ; RETENTION ; GLUTAMATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262795600016 |
| 公开日期 | 2013-12-11 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6787]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 2.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 4.Beijing Inst Technol, Sch Chem Engn & Environm, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wenfang,Zhang, Songping,Wang, Ping. Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration[J]. JOURNAL OF BIOTECHNOLOGY,2009,139(1):102-107. |
| APA | Liu, Wenfang,Zhang, Songping,&Wang, Ping.(2009).Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration.JOURNAL OF BIOTECHNOLOGY,139(1),102-107. |
| MLA | Liu, Wenfang,et al."Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration".JOURNAL OF BIOTECHNOLOGY 139.1(2009):102-107. |
入库方式： OAI收割
来源：过程工程研究所
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