Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography
文献类型：期刊论文
| 作者 | Tan, Tianwei3; Su, Zhi-Guo2; Gu, Ming2; Xu, Jun3; Janson, Jan-Christer1 |
| 刊名 | BIOTECHNOLOGY JOURNAL
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 5期号:5页码:505-510 |
| 关键词 | Cross-linked agarose Green tea catechin HILIC Mixed-mode separation Polyphenol separation |
| ISSN号 | 1860-6768 |
| 其他题名 | Biotechnol. J. |
| 中文摘要 | Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents. |
| 英文摘要 | Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | BOND ADSORPTION CHROMATOGRAPHY ; ONE-STEP PURIFICATION ; 12-PERCENT AGAROSE ; GEL-FILTRATION ; MEDIA ; PARTICLES ; GALLATE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000278461800011 |
| 公开日期 | 2013-11-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6291]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Uppsala Univ, Uppsala Biomed Ctr, Dept Phys & Analyt Chem, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Tianwei,Su, Zhi-Guo,Gu, Ming,et al. Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography[J]. BIOTECHNOLOGY JOURNAL,2010,5(5):505-510. |
| APA | Tan, Tianwei,Su, Zhi-Guo,Gu, Ming,Xu, Jun,&Janson, Jan-Christer.(2010).Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography.BIOTECHNOLOGY JOURNAL,5(5),505-510. |
| MLA | Tan, Tianwei,et al."Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography".BIOTECHNOLOGY JOURNAL 5.5(2010):505-510. |
入库方式： OAI收割
来源：过程工程研究所
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