EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES
文献类型：期刊论文
| 作者 | Liu, Yang1,2,3; Xu, Xia1,4; Ma, Xuehu3; Liu, Jing2; Cui, Zhanfeng1 |
| 刊名 | CRYOLETTERS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 32期号:5页码:425-435 |
| 关键词 | cryopreservation mesenchymal stem cells well defined freezing solutions |
| ISSN号 | 0143-2044 |
| 其他题名 | CryoLetters |
| 中文摘要 | The objective of this study is to compare the effects of different well defined freezing solutions with a reduced concentration of dimethylsulfoxide (DMSO) combined with polyethylene glycol (PEG) and/or trehalose on cryopreservation of mesenchymal stem cells (MSCs) from mice, rats and calves. Post-thaw cell viability, proliferation capacity and differentiation potential of MSCs from different species were assessed after cryopreservation with the conventional slow freezing method. Although the post-thaw viabilities and metabolic activities varied among the different species, satisfactory results were obtained with 5% (v/v) DMSO, 2% (w/v) PEG, 3% (w/v) trehalose and 2% (w/v) bovine serum albumin (BSA) as the freezing solution. Our results showed that mouse MSCs were more robust to cryopreservation compared with rat and bovine MSCs. |
| 英文摘要 | The objective of this study is to compare the effects of different well defined freezing solutions with a reduced concentration of dimethylsulfoxide (DMSO) combined with polyethylene glycol (PEG) and/or trehalose on cryopreservation of mesenchymal stem cells (MSCs) from mice, rats and calves. Post-thaw cell viability, proliferation capacity and differentiation potential of MSCs from different species were assessed after cryopreservation with the conventional slow freezing method. Although the post-thaw viabilities and metabolic activities varied among the different species, satisfactory results were obtained with 5% (v/v) DMSO, 2% (w/v) PEG, 3% (w/v) trehalose and 2% (w/v) bovine serum albumin (BSA) as the freezing solution. Our results showed that mouse MSCs were more robust to cryopreservation compared with rat and bovine MSCs. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology ; Physiology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics ; Physiology |
| 关键词[WOS] | BONE-MARROW ; VIABILITY ; PERMEABILITY ; TREHALOSE ; RECOVERY ; RABBIT ; TISSUE ; SPERM ; SIZE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000296681200007 |
| 公开日期 | 2013-11-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6312]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Oxford, Dept Engn Sci, Inst Biomed Engn, Oxford OX1 3PJ, England 2.Dalian Med Univ, Affiliated Hosp 1, Sino UK Regenerat Med Ctr, Dalian 116011, Peoples R China 3.Dalian Univ Technol, Sch Chem Engn, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yang,Xu, Xia,Ma, Xuehu,et al. EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES[J]. CRYOLETTERS,2011,32(5):425-435. |
| APA | Liu, Yang,Xu, Xia,Ma, Xuehu,Liu, Jing,&Cui, Zhanfeng.(2011).EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES.CRYOLETTERS,32(5),425-435. |
| MLA | Liu, Yang,et al."EFFECT OF VARIOUS FREEZING SOLUTIONS ON CRYOPRESERVATION OF MESENCHYMAL STEM CELLS FROM DIFFERENT ANIMAL SPECIES".CRYOLETTERS 32.5(2011):425-435. |
入库方式： OAI收割
来源：过程工程研究所
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