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Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification

文献类型：期刊论文

作者Jiang Yanjun1,2; Wang Qi1; Wang Wenqin1; Zhou Liya1; Gao Jing1
刊名CHINESE JOURNAL OF CATALYSIS
出版日期2012-05-01
卷号33期号:5页码:857-862
关键词cross-linked enzyme aggregate biomimetic silicification lipase Candida sp 99-125 genipin immobilized enzyme
ISSN号0253-9837
其他题名Chin. J. Catal.
中文摘要The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved.
英文摘要The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved.
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Applied ; Chemistry, Physical ; Engineering, Chemical
研究领域[WOS]Chemistry ; Engineering
关键词[WOS]STABILIZATION ; ACYLASE ; LINKING ; BIOCATALYST ; SUPPORTS ; SILICA
收录类别SCI
原文出处://WOS:000304019200017
语种英语
WOS记录号WOS:000304019200017
公开日期2013-11-28
版本出版稿
源URL[http://ir.ipe.ac.cn/handle/122111/6450]  
专题过程工程研究所_研究所（批量导入）
作者单位1.Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Jiang Yanjun,Wang Qi,Wang Wenqin,et al. Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification[J]. CHINESE JOURNAL OF CATALYSIS,2012,33(5):857-862.
APA Jiang Yanjun,Wang Qi,Wang Wenqin,Zhou Liya,&Gao Jing.(2012).Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification.CHINESE JOURNAL OF CATALYSIS,33(5),857-862.
MLA Jiang Yanjun,et al."Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification".CHINESE JOURNAL OF CATALYSIS 33.5(2012):857-862.

入库方式： OAI收割

来源：过程工程研究所

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