A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV
文献类型：期刊论文
| 作者 | Sun, Lijing1,2; Huang, Yongdong1; Zhang, Yan1,2; Meng, Qingqiang1,2; Luo, Jian1; Fan, Bei3; Ma, Guanghui1; Su, Zhiguo1 |
| 刊名 | ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 39期号:2页码:79-86 |
| 关键词 | haptoglobin human plasma fraction IV purification PEG precipitation ion-exchange chromatography |
| ISSN号 | 1073-1199 |
| 其他题名 | Artif. Cells Blood Substit. Biotechnol. |
| 中文摘要 | Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost. |
| 英文摘要 | Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| 关键词[WOS] | ANTIBODY AFFINITY-CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; BORDETELLA-PERTUSSIS ; AMMONIUM-SULFATE ; PRECIPITATION ; HEMOGLOBIN ; SERUM ; POLYMORPHISM ; TOXIN |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000288022100004 |
| 公开日期 | 2013-11-28 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/6480]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China 3.Hualan Biol Engn Inc, Xinxiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lijing,Huang, Yongdong,Zhang, Yan,et al. A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2011,39(2):79-86. |
| APA | Sun, Lijing.,Huang, Yongdong.,Zhang, Yan.,Meng, Qingqiang.,Luo, Jian.,...&Su, Zhiguo.(2011).A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,39(2),79-86. |
| MLA | Sun, Lijing,et al."A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 39.2(2011):79-86. |
入库方式： OAI收割
来源：过程工程研究所
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