中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 水环境工程研究中心
  5. 期刊论文
Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission

文献类型：期刊论文

作者Qiao, Liang1; Luo, Sang1; Liu, Yongding2; Li, Xiaoyan2; Wang, Gaohong2; Huang, Zebo1
刊名ASTROBIOLOGY
出版日期2013-07-01
卷号13期号:7页码:617-625
关键词Spaceflight Hypergravity Microgravity Caenorhabditis elegans Behavior Reproduction
ISSN号1531-1074
通讯作者Wang, GH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.
英文摘要Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
研究领域[WOS]Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
关键词[WOS]LOW-EARTH-ORBIT ; RADIATION ; SPACE ; NEMATODES ; BEHAVIOR ; DESICCATION ; STRESS ; CELLS
收录类别SCI
资助信息Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources
语种英语
WOS记录号WOS:000321896100003
公开日期2014-01-06
源URL[http://ir.ihb.ac.cn/handle/342005/19597]  
专题水生生物研究所_水环境工程研究中心_期刊论文
作者单位1.Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Qiao, Liang,Luo, Sang,Liu, Yongding,et al. Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission[J]. ASTROBIOLOGY,2013,13(7):617-625.
APA Qiao, Liang,Luo, Sang,Liu, Yongding,Li, Xiaoyan,Wang, Gaohong,&Huang, Zebo.(2013).Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission.ASTROBIOLOGY,13(7),617-625.
MLA Qiao, Liang,et al."Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission".ASTROBIOLOGY 13.7(2013):617-625.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。