Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro
文献类型：期刊论文
| 作者 | Yang, Fangxing1; Zhuang, Shulin1; Zhang, Chao2; Dai, Heping2; Liu, Weiping1 |
| 刊名 | TOXICOLOGY AND APPLIED PHARMACOLOGY
 |
| 出版日期 | 2013-06-15 |
| 卷号 | 269期号:3页码:226-232 |
| 关键词 | Sulforaphane 2 CYP1A1 activity H4IIE cell Genotoxicity Yeast 3 7 8-Tetrachlorodibenzo-p-dioxin |
| ISSN号 | 0041-008X |
| 通讯作者 | Yang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, 388 Yuhangtang Rd, Hangzhou 310058, Peoples R China. |
| 中文摘要 | Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved. |
| 英文摘要 | Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | ARYL-HYDROCARBON RECEPTOR ; SACCHAROMYCES-CEREVISIAE ; POLYCHLORINATED BIPHENYL ; TESTING SYSTEM ; CANCER-RISK ; CELLS ; EXPRESSION ; YEAST ; TCDD ; ETHOXYRESORUFIN |
| 收录类别 | SCI |
| 资助信息 | National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] |
| 语种 | 英语 |
| WOS记录号 | WOS:000319373100003 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19614]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Fangxing,Zhuang, Shulin,Zhang, Chao,et al. Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,269(3):226-232. |
| APA | Yang, Fangxing,Zhuang, Shulin,Zhang, Chao,Dai, Heping,&Liu, Weiping.(2013).Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro.TOXICOLOGY AND APPLIED PHARMACOLOGY,269(3),226-232. |
| MLA | Yang, Fangxing,et al."Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro".TOXICOLOGY AND APPLIED PHARMACOLOGY 269.3(2013):226-232. |
入库方式： OAI收割
来源：水生生物研究所
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