Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR
文献类型：期刊论文
| 作者 | Li, Huiying; Cai, Yan; Xie, Ping; Li, Guangyu; Hao, Le; Xiong, Qian |
| 刊名 | ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 65期号:3页码:537-545 |
| 关键词 | INTERLEUKIN-8 GENE-EXPRESSION PRO-INFLAMMATORY CYTOKINES TROUT ONCORHYNCHUS-MYKISS 3'-UNTRANSLATED REGION REAL-TIME IN-VIVO CHEMOKINES IL-1-BETA CLONING MACROPHAGES |
| ISSN号 | 0090-4341 |
| 通讯作者 | Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp. |
| 英文摘要 | Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | INTERLEUKIN-8 GENE-EXPRESSION ; PRO-INFLAMMATORY CYTOKINES ; TROUT ONCORHYNCHUS-MYKISS ; 3'-UNTRANSLATED REGION ; REAL-TIME ; IN-VIVO ; CHEMOKINES ; IL-1-BETA ; CLONING ; MACROPHAGES |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000324074200017 |
| 公开日期 | 2014-01-03 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19543]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Huiying,Cai, Yan,Xie, Ping,et al. Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR[J]. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2013,65(3):537-545. |
| APA | Li, Huiying,Cai, Yan,Xie, Ping,Li, Guangyu,Hao, Le,&Xiong, Qian.(2013).Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR.ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,65(3),537-545. |
| MLA | Li, Huiying,et al."Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR".ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 65.3(2013):537-545. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。