How much metagenomic sequencing is enough to achieve a given goal?
文献类型：期刊论文
| 作者 | Ni, Jiajia1,2; Yan, Qingyun1; Yu, Yuhe1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2013-06-11 |
| 卷号 | 3期号:- |
| 关键词 | MICROBIAL DIVERSITY GUT MICROBIOME DEEP-SEA BIOSPHERE COMMUNITIES |
| ISSN号 | 2045-2322 |
| 通讯作者 | Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. |
| 中文摘要 | Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal. |
| 英文摘要 | Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MICROBIAL DIVERSITY ; GUT MICROBIOME ; DEEP-SEA ; BIOSPHERE ; COMMUNITIES |
| 收录类别 | SCI |
| 资助信息 | Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] |
| 语种 | 英语 |
| WOS记录号 | WOS:000320130300007 |
| 公开日期 | 2014-01-03 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19556]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, Jiajia,Yan, Qingyun,Yu, Yuhe. How much metagenomic sequencing is enough to achieve a given goal?[J]. SCIENTIFIC REPORTS,2013,3(-). |
| APA | Ni, Jiajia,Yan, Qingyun,&Yu, Yuhe.(2013).How much metagenomic sequencing is enough to achieve a given goal?.SCIENTIFIC REPORTS,3(-). |
| MLA | Ni, Jiajia,et al."How much metagenomic sequencing is enough to achieve a given goal?".SCIENTIFIC REPORTS 3.-(2013). |
入库方式： OAI收割
来源：水生生物研究所
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