UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria
文献类型：期刊论文
| 作者 | Ding, Yi1,2; Song, Lirong1; Sedmak, Bojan3 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-09-13 |
| 卷号 | 8期号:9页码:e73919 |
| 关键词 | ULTRAVIOLET-RADIATION LIGHT AERUGINOSA SOLAR BIOSYNTHESIS EVOLUTION GROWTH DAMAGE GENES |
| ISSN号 | 1932-6203 |
| 通讯作者 | Sedmak, B (reprint author), Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia. |
| 中文摘要 | Due to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280-320nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m(2) in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years' continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers. |
| 英文摘要 | Due to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280-320nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m(2) in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years' continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ULTRAVIOLET-RADIATION ; LIGHT ; AERUGINOSA ; SOLAR ; BIOSYNTHESIS ; EVOLUTION ; GROWTH ; DAMAGE ; GENES |
| 收录类别 | SCI |
| 资助信息 | Slovenian Research Agency |
| 语种 | 英语 |
| WOS记录号 | WOS:000324408400043 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19624]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia |
| 推荐引用方式 GB/T 7714 | Ding, Yi,Song, Lirong,Sedmak, Bojan. UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria[J]. PLOS ONE,2013,8(9):e73919. |
| APA | Ding, Yi,Song, Lirong,&Sedmak, Bojan.(2013).UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria.PLOS ONE,8(9),e73919. |
| MLA | Ding, Yi,et al."UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria".PLOS ONE 8.9(2013):e73919. |
入库方式： OAI收割
来源：水生生物研究所
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