The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China
文献类型：期刊论文
| 作者 | Wang, Zhongjie1,2; Liu, Yang1,2; Xu, Yao1; Xiao, Peng1,2; Li, Renhui1 |
| 刊名 | PHYCOLOGIA
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 52期号:5页码:447-454 |
| 关键词 | cpcBA-IGS Cyanobacteria Dolichospermum flos-aquae Real-time PCR Taihu Lake |
| ISSN号 | 0031-8884 |
| 通讯作者 | Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China. |
| 中文摘要 | The occurrence of bloom-forming heterocystous cyanobacteria such as Dolichospermum flos-aquae has become increasingly serious. This study was conducted to provide a detailed understanding of diversity, distribution and seasonal dynamics of Dolichospermum flos-aquae populations in Taihu Lake, China. Morphological identifications showed Dolichospermum flos-aquae and Aphanizomnon flos-aquae were the two main heterocystous cyanobacteria in Taihu Lake. Phylogenetic analysis based on cpcBA-IGS sequences, the highly variable intergenic spacer (IGS) region between two phycobilisome subunits (cpcB and cpcA), distinguished Dolichospermum from other filametous cyanobacterial genera. Therefore, the real-time PCR detection of Dolichospermum flos-aquae based on cpcBA-IGS sequences was developed to study the distribution and abundance of Dolichospermum flos-aquae. The investigation began in August 2008 and extended through September 2010. Dolichospermum flos-aquae occurred throughout the entire lake. There was an average abundance of 2.0 x 10(6) gene copies L-1, and the peak abundance was in the western part of Taihu Lake during late spring when it reached 4.0 x 10(7) copies L-1. Dolichospermum flos-aquae had a regular annual cycle; it began appearing in late autumn and it reached a peak in late spring. |
| 英文摘要 | The occurrence of bloom-forming heterocystous cyanobacteria such as Dolichospermum flos-aquae has become increasingly serious. This study was conducted to provide a detailed understanding of diversity, distribution and seasonal dynamics of Dolichospermum flos-aquae populations in Taihu Lake, China. Morphological identifications showed Dolichospermum flos-aquae and Aphanizomnon flos-aquae were the two main heterocystous cyanobacteria in Taihu Lake. Phylogenetic analysis based on cpcBA-IGS sequences, the highly variable intergenic spacer (IGS) region between two phycobilisome subunits (cpcB and cpcA), distinguished Dolichospermum from other filametous cyanobacterial genera. Therefore, the real-time PCR detection of Dolichospermum flos-aquae based on cpcBA-IGS sequences was developed to study the distribution and abundance of Dolichospermum flos-aquae. The investigation began in August 2008 and extended through September 2010. Dolichospermum flos-aquae occurred throughout the entire lake. There was an average abundance of 2.0 x 10(6) gene copies L-1, and the peak abundance was in the western part of Taihu Lake during late spring when it reached 4.0 x 10(7) copies L-1. Dolichospermum flos-aquae had a regular annual cycle; it began appearing in late autumn and it reached a peak in late spring. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 研究领域[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 关键词[WOS] | REAL-TIME PCR ; LARGE SHALLOW LAKE ; ENVIRONMENTAL-FACTORS ; MICROCYSTIS BLOOMS ; ANATOXIN-A ; ANABAENA ; WATER ; PHOSPHORUS ; TOXICITY |
| 收录类别 | SCI |
| 资助信息 | NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] |
| 语种 | 英语 |
| WOS记录号 | WOS:000325965100007 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19625]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhongjie,Liu, Yang,Xu, Yao,et al. The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China[J]. PHYCOLOGIA,2013,52(5):447-454. |
| APA | Wang, Zhongjie,Liu, Yang,Xu, Yao,Xiao, Peng,&Li, Renhui.(2013).The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China.PHYCOLOGIA,52(5),447-454. |
| MLA | Wang, Zhongjie,et al."The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China".PHYCOLOGIA 52.5(2013):447-454. |
入库方式： OAI收割
来源：水生生物研究所
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