Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria
文献类型：期刊论文
| 作者 | Zhu, T.; Xu, X. |
| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 57期号:3页码:174-180 |
| 关键词 | acquired chill-light tolerance dual fluorescence assay overwintering cyanobacteria RNA-binding protein 1 viability |
| ISSN号 | 0266-8254 |
| 通讯作者 | Xu, X (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China. |
| 中文摘要 | Chill in the light is the major environmental stress that cyanobacteria encounter in winter. Cyanobacterial cells may acquire chill-light tolerance upon exposure to low temperature in autumn and early winter. We sought to establish the efficacy of the dual fluorescence method in detecting the viability of overwintering cyanobacteria and to provide further evidence for the chill-light tolerance of preconditioned cyanobacteria. Synechocystis sp. PCC 6803 and Microcystis aeruginosa PCC 7806 were exposed to chill (5 degrees C)-light stress with or without pretreatment at 15 degrees C and stained with SYTO 9 and propidium iodide. Live and dead cells were observed under a fluorescence microscope, and the percentage of viable cells was quantified on a microplate reader. The dual fluorescence method showed consistent results with tests of the ability to reinitiate growth. Cell viability was quantitatively correlated with ratio of SYTO 9/propidium iodide fluorescence. Previously, Microcystis colonies in Lake Taihu had been found to accumulate RNA-binding protein 1 in autumn and winter. Use of this method directly showed the viability of such Microcystis colonies throughout the winter. |
| 英文摘要 | Chill in the light is the major environmental stress that cyanobacteria encounter in winter. Cyanobacterial cells may acquire chill-light tolerance upon exposure to low temperature in autumn and early winter. We sought to establish the efficacy of the dual fluorescence method in detecting the viability of overwintering cyanobacteria and to provide further evidence for the chill-light tolerance of preconditioned cyanobacteria. Synechocystis sp. PCC 6803 and Microcystis aeruginosa PCC 7806 were exposed to chill (5 degrees C)-light stress with or without pretreatment at 15 degrees C and stained with SYTO 9 and propidium iodide. Live and dead cells were observed under a fluorescence microscope, and the percentage of viable cells was quantified on a microplate reader. The dual fluorescence method showed consistent results with tests of the ability to reinitiate growth. Cell viability was quantitatively correlated with ratio of SYTO 9/propidium iodide fluorescence. Previously, Microcystis colonies in Lake Taihu had been found to accumulate RNA-binding protein 1 in autumn and winter. Use of this method directly showed the viability of such Microcystis colonies throughout the winter. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | CHILL-LIGHT TOLERANCE ; FLOW-CYTOMETRIC ASSESSMENT ; BACTERIAL VIABILITY ; DRINKING-WATER ; MICROCYSTIS ; MICROORGANISMS ; RHODAMINE-123 ; PCC-6803 ; CULTURES ; LAKE |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] |
| 语种 | 英语 |
| WOS记录号 | WOS:000322954700002 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19639]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, T.,Xu, X.. Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria[J]. LETTERS IN APPLIED MICROBIOLOGY,2013,57(3):174-180. |
| APA | Zhu, T.,&Xu, X..(2013).Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria.LETTERS IN APPLIED MICROBIOLOGY,57(3),174-180. |
| MLA | Zhu, T.,et al."Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria".LETTERS IN APPLIED MICROBIOLOGY 57.3(2013):174-180. |
入库方式： OAI收割
来源：水生生物研究所
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