Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)
文献类型：期刊论文
| 作者 | Chen, Zhongyuan1; Gui, Jianfang1; Gao, Xiaochan1; Pei, Chao1; Hong, Yijiang2; Zhang, Qiya1 |
| 刊名 | VETERINARY RESEARCH
 |
| 出版日期 | 2013-10-21 |
| 卷号 | 44页码:101 |
| 关键词 | RANA-GRYLIO VIRUS FISH CELL-LINE FAMILY-IRIDOVIRIDAE VIRAL REPLICATION NUCLEAR IMPORT FROG SEQUENCE PROTEIN EXPORT NUCLEOPROTEIN |
| ISSN号 | 0928-4249 |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses. |
| 英文摘要 | Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Veterinary Sciences |
| 研究领域[WOS] | Veterinary Sciences |
| 关键词[WOS] | RANA-GRYLIO VIRUS ; FISH CELL-LINE ; FAMILY-IRIDOVIRIDAE ; VIRAL REPLICATION ; NUCLEAR IMPORT ; FROG ; SEQUENCE ; PROTEIN ; EXPORT ; NUCLEOPROTEIN |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] |
| 语种 | 英语 |
| WOS记录号 | WOS:000326186900001 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19570]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Nanchang Univ, Coll Life Sci & Food Engn, Nanchang 330031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhongyuan,Gui, Jianfang,Gao, Xiaochan,et al. Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)[J]. VETERINARY RESEARCH,2013,44:101. |
| APA | Chen, Zhongyuan,Gui, Jianfang,Gao, Xiaochan,Pei, Chao,Hong, Yijiang,&Zhang, Qiya.(2013).Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV).VETERINARY RESEARCH,44,101. |
| MLA | Chen, Zhongyuan,et al."Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)".VETERINARY RESEARCH 44(2013):101. |
入库方式： OAI收割
来源：水生生物研究所
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