Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs
文献类型：期刊论文
| 作者 | Liu, Yun1,4; Luo, Daji2,3; Zhao, Hui1,4; Zhu, Zuoyan2; Hu, Wei2; Cheng, Christopher H. K.1,4 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-10-10 |
| 卷号 | 8期号:10页码:e76387 |
| 关键词 | ZINC-FINGER NUCLEASES EFFECTOR NUCLEASES CHROMOSOMAL DELETIONS EMBRYONIC-DEVELOPMENT MICRORNA EXPRESSION TRANSCRIPTION CELLS KNOCKOUT ORGANIZATION RECOGNITION |
| ISSN号 | 1932-6203 |
| 通讯作者 | Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China. |
| 中文摘要 | Transcription activator-like effector nucleases (TALENs) have so far been applied to disrupt protein-coding genes which constitute only 2-3% of the genome in animals. The majority (70-90%) of the animal genome is actually transcribed as non-coding RNAs (ncRNAs), yet the lack of efficient tools to knockout ncRNA genes hinders studies on their in vivo functions. Here we have developed novel strategies using TALENs to achieve precise and inheritable large genomic deletions and knockout of ncRNA genes in zebrafish. We have demonstrated that individual miRNA genes could be disrupted using one pair of TALENs, whereas large microRNA (miRNA) gene clusters and long non-coding RNA (lncRNA) genes could be precisely deleted using two pairs of TALENs. We have generated large genomic deletions of two miRNA clusters (the 1.2 kb miR-17-92 cluster and the 79.8 kb miR-430 cluster) and one long non-coding RNA (lncRNA) gene (the 9.0 kb malat1), and the deletions are transmitted through the germline. Taken together, our results establish TALENs as a robust tool to engineer large genomic deletions and knockout of ncRNA genes, thus opening up new avenues in the application of TALENs to study the genome in vivo. |
| 英文摘要 | Transcription activator-like effector nucleases (TALENs) have so far been applied to disrupt protein-coding genes which constitute only 2-3% of the genome in animals. The majority (70-90%) of the animal genome is actually transcribed as non-coding RNAs (ncRNAs), yet the lack of efficient tools to knockout ncRNA genes hinders studies on their in vivo functions. Here we have developed novel strategies using TALENs to achieve precise and inheritable large genomic deletions and knockout of ncRNA genes in zebrafish. We have demonstrated that individual miRNA genes could be disrupted using one pair of TALENs, whereas large microRNA (miRNA) gene clusters and long non-coding RNA (lncRNA) genes could be precisely deleted using two pairs of TALENs. We have generated large genomic deletions of two miRNA clusters (the 1.2 kb miR-17-92 cluster and the 79.8 kb miR-430 cluster) and one long non-coding RNA (lncRNA) gene (the 9.0 kb malat1), and the deletions are transmitted through the germline. Taken together, our results establish TALENs as a robust tool to engineer large genomic deletions and knockout of ncRNA genes, thus opening up new avenues in the application of TALENs to study the genome in vivo. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ZINC-FINGER NUCLEASES ; CHROMOSOMAL DELETIONS ; EFFECTOR NUCLEASES ; EMBRYONIC-DEVELOPMENT ; MICRORNA EXPRESSION ; TRANSCRIPTION ; CELLS ; KNOCKOUT ; ORGANIZATION ; RECOGNITION |
| 收录类别 | SCI |
| 资助信息 | Research Grant Council of Hong Kong [462312]; National Natural Science Foundation of China [31172394, 30900853, 30930069]; Chinese Academy of Sciences [KSCX2-EW-N-004] |
| 语种 | 英语 |
| WOS记录号 | WOS:000325814200038 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19571]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Univ Hong Kong, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 3.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Peoples R China 4.Chinese Univ Hong Kong, Shenzhen Res Inst, Sch Biomed Sci, Core Lab, Shenzhen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yun,Luo, Daji,Zhao, Hui,et al. Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs[J]. PLOS ONE,2013,8(10):e76387. |
| APA | Liu, Yun,Luo, Daji,Zhao, Hui,Zhu, Zuoyan,Hu, Wei,&Cheng, Christopher H. K..(2013).Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs.PLOS ONE,8(10),e76387. |
| MLA | Liu, Yun,et al."Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs".PLOS ONE 8.10(2013):e76387. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。