Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells
文献类型：期刊论文
| 作者 | Zhu, Rong; Wang, Jun; Lei, Xiao-Ying; Gui, Jian-Fang; Zhang, Qi-Ya |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 35期号:3页码:918-926 |
| 关键词 | Paralichthys olivaceus interferon-inducible transmembrane 1 (PoIFITM1) Antiviral effect Rana grylio virus (RGV) Scophthalmus maximus rhabdovirus (SMRV) Golgi localization |
| ISSN号 | 1050-4648 |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China. |
| 中文摘要 | Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | VIRUS-REPLICATION ; S-PALMITOYLATION ; INTERFERON ; INFLUENZA ; PROTEINS ; EXPRESSION ; IRIDOVIRUS ; RESISTANCE ; CLONING ; GENES |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] |
| 语种 | 英语 |
| WOS记录号 | WOS:000324511700035 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19576]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Rong,Wang, Jun,Lei, Xiao-Ying,et al. Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):918-926. |
| APA | Zhu, Rong,Wang, Jun,Lei, Xiao-Ying,Gui, Jian-Fang,&Zhang, Qi-Ya.(2013).Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells.FISH & SHELLFISH IMMUNOLOGY,35(3),918-926. |
| MLA | Zhu, Rong,et al."Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):918-926. |
入库方式： OAI收割
来源：水生生物研究所
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