Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)
文献类型：期刊论文
| 作者 | Zhu, Chuankun1,2; Yu, Xiaomu1; Fu, B.1; Tong, Jingou1 |
| 刊名 | CONSERVATION GENETICS RESOURCES
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 5期号:3页码:755-758 |
| 关键词 | Bighead carp (Aristichthys nobilis) Microsatellite Polymorphism Silver carp (Hypophthalmichthys molitrix) Cross-species amplification |
| ISSN号 | 1877-7252 |
| 通讯作者 | Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp. |
| 英文摘要 | In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biodiversity Conservation ; Genetics & Heredity |
| 研究领域[WOS] | Biodiversity & Conservation ; Genetics & Heredity |
| 收录类别 | SCI |
| 资助信息 | MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] |
| 语种 | 英语 |
| WOS记录号 | WOS:000322619900037 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19578]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Chuankun,Yu, Xiaomu,Fu, B.,et al. Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)[J]. CONSERVATION GENETICS RESOURCES,2013,5(3):755-758. |
| APA | Zhu, Chuankun,Yu, Xiaomu,Fu, B.,&Tong, Jingou.(2013).Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis).CONSERVATION GENETICS RESOURCES,5(3),755-758. |
| MLA | Zhu, Chuankun,et al."Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)".CONSERVATION GENETICS RESOURCES 5.3(2013):755-758. |
入库方式： OAI收割
来源：水生生物研究所
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