Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing
文献类型：期刊论文
| 作者 | Wu, Shan-Gong1; Tian, Jing-Yun2; Gatesoupe, Francois-Joel3; Li, Wen-Xiang1; Zou, Hong1; Yang, Bao-Juan1; Wang, Gui-Tang1 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2013-09-01 |
| 卷号 | 29期号:9页码:1585-1595 |
| 关键词 | Gibel carp Intestine Microbiota Pyrosequencing |
| ISSN号 | 0959-3993 |
| 通讯作者 | Wang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China. |
| 中文摘要 | The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics. |
| 英文摘要 | The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | BACTERIAL-POPULATIONS ; GUT MICROBIOTA ; DIVERSITY ; IDENTIFICATION ; FISH ; AMPLIFICATION ; MICROFLORA ; LARVAE ; MUCUS ; DNA |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] |
| 语种 | 英语 |
| WOS记录号 | WOS:000323223300005 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19579]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China 3.IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France |
| 推荐引用方式 GB/T 7714 | Wu, Shan-Gong,Tian, Jing-Yun,Gatesoupe, Francois-Joel,et al. Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(9):1585-1595. |
| APA | Wu, Shan-Gong.,Tian, Jing-Yun.,Gatesoupe, Francois-Joel.,Li, Wen-Xiang.,Zou, Hong.,...&Wang, Gui-Tang.(2013).Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(9),1585-1595. |
| MLA | Wu, Shan-Gong,et al."Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.9(2013):1585-1595. |
入库方式： OAI收割
来源：水生生物研究所
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