Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories
文献类型：期刊论文
| 作者 | Ke, Fei; He, Li-Bo; Zhang, Qi-Ya |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013-05-06 |
| 卷号 | 8期号:5页码:e63737 |
| ISSN号 | 1932-6203 |
| 关键词 | RANA-GRYLIO-VIRUS GRASS CARP REOVIRUS MU-NS SIGMA-NS FISH CELLS PARALICHTHYS-OLIVACEUS FUNCTIONAL DOMAINS GENUS AQUAREOVIRUS FAMILY REOVIRIDAE INCLUSIONS |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China. |
| 中文摘要 | Background: Replication and assembly of vertebrate reoviruses occur in specific intracellular compartments known as viral factories. Recently, NS88 and NS80, the nonstructural proteins from aquareoviruses, have been proposed to share common traits with mu NS from orthoreoviruses, which are involved in the formation of viral factories. Methodology/Principal Findings: In this study, the NS80 characteristics and its interactions with other viral components were investigated. We observed that the NS80 structure ensured its self-aggregation and selective recruitment of viral proteins to viral factories like structures (VFLS). The minimum amino acids (aa) of NS80 required for VFLS formation included 193 aa at the C-terminal. However, this truncated protein only contained one aa coil and located in the nucleus. Its N-terminal residual regions, aa 1-55 and aa 55-85, were required for recruiting viral nonstructural protein NS38 and structural protein VP3, respectively. A conserved N-terminal region of NS38, which was responsible for the interaction with NS80, was also identified. Moreover, the minimal region of C-terminal residues, aa 506-742 (Delta 505), required for NS80 self-aggregation in the cytoplasm, and aa 550-742 (Delta 549), which are sufficient for recruiting viral structure proteins VP1, VP2, and VP4 were also identified. Conclusions/Significance: The present study shows detailed interactions between NS80 and NS38 or other viral proteins. Sequence and structure characteristics of NS80 ensures its self-aggregation to form VFLS (either in the cytoplasm or nucleus) and recruitment of viral structural or nonstructural proteins. |
| 英文摘要 | Background: Replication and assembly of vertebrate reoviruses occur in specific intracellular compartments known as viral factories. Recently, NS88 and NS80, the nonstructural proteins from aquareoviruses, have been proposed to share common traits with mu NS from orthoreoviruses, which are involved in the formation of viral factories. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | RANA-GRYLIO-VIRUS ; GRASS CARP REOVIRUS ; MU-NS ; SIGMA-NS ; FISH CELLS ; PARALICHTHYS-OLIVACEUS ; FUNCTIONAL DOMAINS ; GENUS AQUAREOVIRUS ; FAMILY REOVIRIDAE ; INCLUSIONS |
| 资助信息 | National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321390200059 |
| 公开日期 | 2014-01-06 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19586]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Fei,He, Li-Bo,Zhang, Qi-Ya. Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories[J]. PLOS ONE,2013,8(5):e63737. |
| APA | Ke, Fei,He, Li-Bo,&Zhang, Qi-Ya.(2013).Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories.PLOS ONE,8(5),e63737. |
| MLA | Ke, Fei,et al."Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories".PLOS ONE 8.5(2013):e63737. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。