Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings
文献类型：期刊论文
| 作者 | Deng, Wei-Wei1,2; Fei, Yue1,2; Wang, Shuo1,2; Wan, Xiao-Chun1,2; Zhang, Zheng-Zhu1,2; Hu, Xiang-Yang3 |
| 刊名 | PLANT GROWTH REGULATION
 |
| 出版日期 | 2013-12-01 |
| 卷号 | 71期号:3页码:295-299 |
| 关键词 | Camellia sinensis Theanine synthetase Shade Theanine Amino acid |
| ISSN号 | 0167-6903 |
| 通讯作者 | Zhang,ZZ (reprint author),Anhui Agr Univ,Key Lab Tea Biochem & Biotechnol,Minist Educ,130 Changjiang West Rd,Hefei 230036,Anhui,Peoples R China.,zzz@ahau.edu.cn |
| 英文摘要 | Theanine synthetase (TS) is a key enzyme involved in theanine biosynthesis. In our recent study, it has been revealed that theanine biosynthesis derived from nitrogen metabolism in tea (Camellia sinensis) plants can be influenced by shading treatment. The expression patterns of CsTS protein in the roots and shoots of tea seedlings were examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of long-term shade treatment on the levels of theanine synthesis was also investigated using roots and shoots of tea seedlings. Levels of theanine and total free amino acids gradually increased in shoots, reaching their maximum after 22 days of treatment (DOT). The immunoblotting analysis suggested that CsTS protein levels increased gradually up to 22 DOT and expression remained at a high level, except after 1 DOT where levels were low in both roots and shoots. The increased theanine concentration we observed in the shading treatment may be due to increased nitrogen assimilation and reduced theanine catabolism under shade conditions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Plant Sciences |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; FREE AMINO-ACIDS ; TEA PLANTS ; METABOLISM ; EXPRESSION |
| 收录类别 | SCI |
| 资助信息 | Program for Changjiang Scholars and Innovative Research Team in University [IRT1101];Natural Science Foundation of China [31170283] |
| 语种 | 英语 |
| WOS记录号 | WOS:000326622700010 |
| 公开日期 | 2014-01-13 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/17430]  |
| 专题 | 昆明植物研究所_中国科学院青藏高原研究所昆明部 |
| 作者单位 | 1.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Anhui, Peoples R China 2.Anhui Agr Univ, Minist Agr, Hefei 230036, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Wei-Wei,Fei, Yue,Wang, Shuo,et al. Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings[J]. PLANT GROWTH REGULATION,2013,71(3):295-299. |
| APA | Deng, Wei-Wei,Fei, Yue,Wang, Shuo,Wan, Xiao-Chun,Zhang, Zheng-Zhu,&Hu, Xiang-Yang.(2013).Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings.PLANT GROWTH REGULATION,71(3),295-299. |
| MLA | Deng, Wei-Wei,et al."Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings".PLANT GROWTH REGULATION 71.3(2013):295-299. |
入库方式： OAI收割
来源：昆明植物研究所
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