Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage
文献类型：期刊论文
| 作者 | Zhao, Longyan1,2 ; Lai, Sensen1,3,4; Huang, Rong5; Wu, Mingyi1; Gao, Na1; Xu, Li1,2; Qin, Hongbo1; Peng, Wenlie3; Zhao, Jinhua1
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| 刊名 | CARBOHYDRATE POLYMERS
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| 出版日期 | 2013-11-06 |
| 卷号 | 98期号:2页码:1514-1523 |
| 关键词 | Sea cucumber Glycosaminoglycan Deacetylation Deaminative cleavage Anticoagulant activity |
| ISSN号 | 0144-8617 |
| 通讯作者 | Qin,HB (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,Kunming,Peoples R China.,qinhongbo@mail.kib.ac.cn ; peng-wenlie@21cn.com |
| 英文摘要 | Fucosylated glycosaminoglycans (FGs) are complex glycosaminoglycans that exhibit potent anticoagulant activity. To study the relationship between molecular size and biological activity, oligosaccharides with (2,5)-anhydro-D-talose units at new reducing ends were prepared by hydrazine deacetylation and nitrous acid depolymerization. The product chemical structures were analyzed by one- and two-dimensional NMR methods. Additionally, anticoagulant activities were evaluated by clotting assay and chromogenic substrate cleavage. The results demonstrated that under mild deacetylation and deaminative cleavage conditions, both products were relatively homogeneous and sulfated fucose branch types and sulfate sub-stituents remained stable. These depolymerized FGs with different molecular sizes had potent intrinsic anticoagulant activities, which were similar to those that were obtained by free-radical depolymerization with similar molecular weights. Decreasing molecular weight may weaken activity but not significantly affect factor Xase and heparin cofactor II (HCII)-mediated thrombin inhibition. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Chemistry ; Polymer Science |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Polymer Science |
| 关键词[WOS] | DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN ; NITROUS-ACID DEPOLYMERIZATION ; FREE-RADICAL DEPOLYMERIZATION ; STICHOPUS-JAPONICUS SELENKA ; CUCUMBER THELENATA-ANANAS ; CHONDROITIN SULFATE ; SEA-CUCUMBER ; PHYSICOCHEMICAL CHARACTERISTICS ; HEPARIN ; OLIGOSACCHARIDES |
| 收录类别 | SCI |
| 资助信息 | Yunnan Provincial Science and Technology Department in China [2010CI116, 2012BC011, 2012FB177];National Natural Science Foundation of China [81102372];Outstanding Technical Talent Foundation of the Chinese Academy of Sciences;West Light Foundation of the Chinese Academy of Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000326313600035 |
| 公开日期 | 2014-01-13 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/17420]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Sun Yat Sen Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China 4.Ganzhou Prov Inst Food & Drug Control, Ganzhou, Peoples R China 5.Yunan Univ, Sch Chem Sci & Technol, Minist Educ, Key Lab Med Chem Nat Resource, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Longyan,Lai, Sensen,Huang, Rong,et al. Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage[J]. CARBOHYDRATE POLYMERS,2013,98(2):1514-1523. |
| APA | Zhao, Longyan.,Lai, Sensen.,Huang, Rong.,Wu, Mingyi.,Gao, Na.,...&Zhao, Jinhua.(2013).Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage.CARBOHYDRATE POLYMERS,98(2),1514-1523. |
| MLA | Zhao, Longyan,et al."Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage".CARBOHYDRATE POLYMERS 98.2(2013):1514-1523. |
入库方式： OAI收割
来源：昆明植物研究所
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