A dynamic model for processive transcription elongation and backtracking long pauses by multisubunit RNA polymerases
文献类型：期刊论文
| 作者 | Xie, P |
| 刊名 | PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
 |
| 出版日期 | 2012 |
| 卷号 | 80期号:8页码:2020 |
| 关键词 | 3.3 ANGSTROM RESOLUTION I KLENOW FRAGMENT STRUCTURAL BASIS DNA-POLYMERASE ESCHERICHIA-COLI TEMPERATURE-DEPENDENCE EXONUCLEASE SITES CRYSTAL-STRUCTURE SINGLE-MOLECULE ACTIVE-CENTER |
| ISSN号 | 0887-3585 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Key Lab Soft Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | RNA polymerases are enzymes that transcribe genes from DNA onto strands of RNA. The transcription elongation by multisubunit RNA polymerases is processive but nonuniform: one enzyme can translocate along the DNA template for thousands of nucleotide addition steps but, sometimes, it can enter backtracking long pauses. Here, we present a Brownian ratchet model for the processive transcription elongation and the backtracking long pauses, which is developed based on the available structural and biochemical studies. Using the model, we analytically study the dynamics of the transcription elongation, such as the effects of external force and NTP concentration on the transcription velocity free of pauses, and the dynamics of backtracking long pauses, such as the probabilities of entering and returning from the backtracking pauses, with the analytical results in good agreement with the available single molecule experimental data. Values of several parameters for both Escherichia coli and Saccharomyces cerevisiae RNA polymerases such as their affinities for the DNA/RNA substrate during transcription elongation are determined. Moreover, some testable predictions are presented. Proteins 2012; (c) 2012 Wiley Periodicals, Inc. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10974248] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/32890]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P. A dynamic model for processive transcription elongation and backtracking long pauses by multisubunit RNA polymerases[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,2012,80(8):2020. |
| APA | Xie, P.(2012).A dynamic model for processive transcription elongation and backtracking long pauses by multisubunit RNA polymerases.PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,80(8),2020. |
| MLA | Xie, P."A dynamic model for processive transcription elongation and backtracking long pauses by multisubunit RNA polymerases".PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 80.8(2012):2020. |
入库方式： OAI收割
来源：物理研究所
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