A polymerase-site-jumping model for strand transfer during DNA synthesis by reverse transcriptase
文献类型：期刊论文
| 作者 | Xie, P |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 144期号:1-2页码:65 |
| 关键词 | HUMAN-IMMUNODEFICIENCY-VIRUS MURINE LEUKEMIA-VIRUS HIGH NEGATIVE INTERFERENCE STRONG STOP TRANSFER RNASE-H ACTIVITIES RETROVIRAL RECOMBINATION CRYSTAL-STRUCTURE NUCLEOCAPSID PROTEIN IN-VITRO GENETIC-RECOMBINATION |
| ISSN号 | 0168-1702 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Inst Phys, Lab Saft Matter Phys, Zhongguancun Sourth St 3, Beijing 100190, Peoples R China. |
| 中文摘要 | During reverse transcription, besides the obligatory strand transfers associated with replication at the ends of the viral genome, multiple strand transfers often occur associated with replication within internal regions. Here, based on previous structural and biochemical studies, a model is proposed for processive DNA synthesis along a single template mediated by reverse transcriptase and, based on this model, the mechanism of inter- or intramolecular strand transfers during minus DNA synthesis is presented. A strand-transfer event involves two steps, with the first one being the annealing of the nascent DNA with acceptor RNA at the upstream position of the reverse transcriptase while the second one being the jumping of the polymerase active site to the acceptor. Using the model, the promotion of strand transfer by pausing and high frequent deletions induced by strand transfers can be well explained. We present analytical studies of the efficiency of single strand-transfer event and of the efficiency of multiple-strand-transfer events, with which the high negative interference can be well explained. The dependence of strand-transfer efficiency on the ratio between polymerase and RNase H rates, the role of the polymerase-dependent and polymerase-independent cleavages in strand transfers and the efficiency of nonhomologous strand transfer are analytically studied. The theoretical results are in agreement with the available experimental data. Moreover, some predicted results of the dependence of negative interference on the ratio of polymerase over RNase H rates are presented. (C) 2009 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/33170]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P. A polymerase-site-jumping model for strand transfer during DNA synthesis by reverse transcriptase[J]. VIRUS RESEARCH,2009,144(1-2):65. |
| APA | Xie, P.(2009).A polymerase-site-jumping model for strand transfer during DNA synthesis by reverse transcriptase.VIRUS RESEARCH,144(1-2),65. |
| MLA | Xie, P."A polymerase-site-jumping model for strand transfer during DNA synthesis by reverse transcriptase".VIRUS RESEARCH 144.1-2(2009):65. |
入库方式： OAI收割
来源：物理研究所
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