A possible mechanism for the dynamics of transition between polymerase and exonuclease sites in a high-fidelity DNA polymerase
文献类型：期刊论文
| 作者 | Xie, P |
| 刊名 | JOURNAL OF THEORETICAL BIOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 259期号:3页码:434 |
| 关键词 | SINGLE-STRANDED-DNA I KLENOW FRAGMENT CONFERS PROCESSIVITY CRYSTAL-STRUCTURES MOLECULAR MOTORS REPLICATION TEMPLATE COMPLEXES TENSION BINDING |
| ISSN号 | 0022-5193 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100790, Peoples R China. |
| 中文摘要 | The fidelity of DNA synthesis by DNA polymerase is significantly increased by a mechanism of proofreading that is performed at the exonuclease active site separate from the polymerase active site. Thus, the transition of DNA between the two active sites is an important activity of DNA polymerase. Here, based on our proposed model, the rates of DNA transition between the two active sites are theoretically studied. With the relevant parameters, which are determined from the available crystal structure and other experimental data, the calculated transfer rate of correctly base-paired DNA from the polymerase to exonuclease sites and the transfer rate after incorporation of a mismatched base are in good agreement with the available experimental data. The transfer rates in the presence of two and three mismatched bases are also consistent with the previous experimental data. In addition, the calculated transfer rate from the exonuclease to polymerase sites has a large value even with the high binding affinity of 3'-5' ssDNA for the exonuclease site, which is also consistent with the available experimental value. Moreover, we also give some predictive results for the transfer rate of DNA containing only A:T base pairs and that of DNA containing only G:C base pairs. (C) 2009 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/33172]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P. A possible mechanism for the dynamics of transition between polymerase and exonuclease sites in a high-fidelity DNA polymerase[J]. JOURNAL OF THEORETICAL BIOLOGY,2009,259(3):434. |
| APA | Xie, P.(2009).A possible mechanism for the dynamics of transition between polymerase and exonuclease sites in a high-fidelity DNA polymerase.JOURNAL OF THEORETICAL BIOLOGY,259(3),434. |
| MLA | Xie, P."A possible mechanism for the dynamics of transition between polymerase and exonuclease sites in a high-fidelity DNA polymerase".JOURNAL OF THEORETICAL BIOLOGY 259.3(2009):434. |
入库方式： OAI收割
来源：物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。