A thermal ratchet model of tRNA-mRNA translocation by the ribosome
文献类型：期刊论文
| 作者 | Xie, P |
| 刊名 | BIOSYSTEMS
 |
| 出版日期 | 2009 |
| 卷号 | 96期号:1页码:19 |
| 关键词 | ELONGATION-FACTOR-G PEPTIDYL-TRANSFER-RNA ESCHERICHIA-COLI 70S RIBOSOME E-SITE P-SITE ANGSTROM RESOLUTION BACTERIAL RIBOSOME PROTEIN-SYNTHESIS CRYSTAL-STRUCTURE |
| ISSN号 | 0303-2647 |
| 通讯作者 | Xie, P: Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | The translocation of tRNA coupled with mRNA in the ribosome is one of important steps during protein synthesis. Despite extensive experimental studies, the detailed mechanism of the translocation remains undetermined. Here, based on previous biochemical, cryo-electron microscopic and X-ray crystallographic studies, a thermal ratchet model is presented for this translocation. In the model, during one elongation cycle of the protein synthesis, two large conformational transitions of the ribosome are involved, with one being the relative rotation between the two ribosomal subunits following the peptide transfer, which is facilitated by the EF-G.GTP binding, and the other one being the reverse relative rotation between the two ribosomal subunits upon EF-G.GTP hydrolysis. The former conformational change plays an important role in ensuring the completion of the release of the deacylated tRNA from the ribosome before tRNA-mRNA translocation. The latter reverse conformational change upon GTP hydrolysis is followed by rapid tRNA-mRNA translocation and Pi release, both of which take place independently of each other. This is consistent with the previous biochemical experimental data. Also, the model is consistent with other available experimental results such as the suppression of EF-G-dependent translocation in cross-linked ribosomes and frameshifting under some conditions. (C) 2008 Elsevier Ireland Ltd. All rights reserved. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/33315]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xie, P. A thermal ratchet model of tRNA-mRNA translocation by the ribosome[J]. BIOSYSTEMS,2009,96(1):19. |
| APA | Xie, P.(2009).A thermal ratchet model of tRNA-mRNA translocation by the ribosome.BIOSYSTEMS,96(1),19. |
| MLA | Xie, P."A thermal ratchet model of tRNA-mRNA translocation by the ribosome".BIOSYSTEMS 96.1(2009):19. |
入库方式： OAI收割
来源：物理研究所
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