An improved loopless mounting method for cryocrystallography
文献类型：期刊论文
| 作者 | Qi, JX ; Jiang, F |
| 刊名 | CHINESE PHYSICS B
 |
| 出版日期 | 2010 |
| 卷号 | 19期号:1 |
| 关键词 | MACROMOLECULAR CRYOCRYSTALLOGRAPHY BIOLOGICAL MACROMOLECULES PROTEIN CRYSTALS CRYSTALLOGRAPHY TEMPERATURE SYSTEM |
| ISSN号 | 1674-1056 |
| 通讯作者 | Jiang, F: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Based on a recent loopless mounting method, a simplified loopless and bufferless crystal mounting method is developed for macromolecular crystallography. This simplified crystal mounting system is composed of the following components: a home-made glass capillary, a brass seat for holding the glass capillary, a flow regulator, and a vacuum pump for evacuation. Compared with the currently prevalent loop mounting method, this simplified method has almost the same mounting procedure and thus is compatible with the current automated crystal mounting system. The advantages of this method include higher signal-to-noise ratio, more accurate measurement, more rapid flash cooling, less x-ray absorption and thus less radiation damage to the crystal. This method can be extended to the flash-freeing of a crystal without or with soaking it in a lower concentration of cryoprotectant, thus it may be the best option for data collection in the absence of suitable cryoprotectant. Therefore, it is suggested that this mounting method should be further improved and extensively applied to cryocrystallographic experiments. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundations of China [10674172] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/33652]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qi, JX,Jiang, F. An improved loopless mounting method for cryocrystallography[J]. CHINESE PHYSICS B,2010,19(1). |
| APA | Qi, JX,&Jiang, F.(2010).An improved loopless mounting method for cryocrystallography.CHINESE PHYSICS B,19(1). |
| MLA | Qi, JX,et al."An improved loopless mounting method for cryocrystallography".CHINESE PHYSICS B 19.1(2010). |
入库方式： OAI收割
来源：物理研究所
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