An improved single molecule manipulation apparatus and its applications
文献类型：期刊论文
| 作者 | Wang, XL ; Zhang, XH ; Wei, KJ ; Sun, B ; Li, M |
| 刊名 | ACTA PHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 57期号:6页码:3905 |
| 关键词 | COLI RNA-POLYMERASE SUPERCOILED DNA TRANSLOCATION TRANSCRIPTION ELASTICITY TENSION FORCE FTSK |
| ISSN号 | 1000-3290 |
| 通讯作者 | Li, M: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | We report an improvement to the lateral-magnetic-tweezers which can be used to stretch and twist DNA molecules, and to probe the interaction between DNA and proteins. The apparatus enables not only direct measurement of the extension and rotation of DNA but also easy refreshing of the buffer in the flow chamber. With this apparatus, the experimental efficiency can be greatly improved by manipulating several DNA molecules simultaneously. The force exerted on DNA molecules ranges from 0.1 to 40 pN, satisfying the requirement of most single molecule experiments. To demonstrate the applicability of the apparatus, we measured the extension-force curve and the extensions rotation curve of DNA and studied the translocation of DNA by a motor protein. The results agree well with those reported in the literature. |
| 收录类别 | SCI |
| 语种 | 中文 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/33653]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, XL,Zhang, XH,Wei, KJ,et al. An improved single molecule manipulation apparatus and its applications[J]. ACTA PHYSICA SINICA,2008,57(6):3905. |
| APA | Wang, XL,Zhang, XH,Wei, KJ,Sun, B,&Li, M.(2008).An improved single molecule manipulation apparatus and its applications.ACTA PHYSICA SINICA,57(6),3905. |
| MLA | Wang, XL,et al."An improved single molecule manipulation apparatus and its applications".ACTA PHYSICA SINICA 57.6(2008):3905. |
入库方式： OAI收割
来源：物理研究所
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