Are Coiled-Coils of Dimeric Kinesins Unwound during Their Walking on Microtubule?
文献类型：期刊论文
| 作者 | Duan, ZW ; Xie, P ; Li, W ; Wang, PY |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:4 |
| 关键词 | HAND-OVER-HAND NCD MOTOR PROTEIN CRYSTAL-STRUCTURE DRIVES MOTILITY YEAST KAR3 NECK MECHANISM MOLECULE MOVEMENT HEAD |
| ISSN号 | 1932-6203 |
| 通讯作者 | Duan, ZW: Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | Dimeric kinesin motor proteins such as homodimeric kinesin-1, homodimeric Ncd and heterodimeric Kar3/Vik1are composed of two head domains which are connected together by a rod-shaped, coiled-coil stalk. Despite the extensive and intensive studies on structures, kinetics, dynamics and walking mechanism of the dimers, whether their coiled-coils are unwound or not during their walking on the microtubule is still an unclear issue. Here, we try to clarify this issue by using molecular dynamics simulations. Our simulation results showed that, for Ncd, a large change in potential of mean force is required to unwind the coiled-coil by only several pairs of residues. For both Ncd and kinesin-1, the force required to initiate the coiled-coil unwinding is larger than that required for unfolding of the single alpha-helix that forms the coiled-coil or is larger than that required to unwind the DNA duplex, which is higher than the unbinding force of the kinesin head from the microtubule in strong microtubule-binding states. Based on these results and the comparison of the sequence between the coiled-coil of Kar3/Vik1 and those of Ncd and kinesin-1, it was deduced that the coiled-coil of the Kar3/Vik1 should also be very stable. Thus, we concluded that the coiled-coils of kinesin-1, Ncd and Kar3/Vik1 are almost impossible to unwind during their walking on the microtubule. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [10974248, 10834014]; National Basic Research Program of China (973 Program) [2009CB930704] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/33969]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Duan, ZW,Xie, P,Li, W,et al. Are Coiled-Coils of Dimeric Kinesins Unwound during Their Walking on Microtubule?[J]. PLOS ONE,2012,7(4). |
| APA | Duan, ZW,Xie, P,Li, W,&Wang, PY.(2012).Are Coiled-Coils of Dimeric Kinesins Unwound during Their Walking on Microtubule?.PLOS ONE,7(4). |
| MLA | Duan, ZW,et al."Are Coiled-Coils of Dimeric Kinesins Unwound during Their Walking on Microtubule?".PLOS ONE 7.4(2012). |
入库方式： OAI收割
来源：物理研究所
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