Cisplatin induces loop structures and condensation of single DNA molecules
文献类型：期刊论文
| 作者 | Hou, XM ; Zhang, XH ; Wei, KJ ; Ji, C ; Dou, SX ; Wang, WC ; Li, M ; Wang, PY |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 37期号:5页码:1400 |
| 关键词 | ANTICANCER DRUG CISPLATIN INTRASTRAND CROSS-LINK ATOMIC-FORCE MICROSCOPY CRYSTAL-STRUCTURE PERSISTENCE LENGTH DODECAMER DUPLEX SUPERCOILED DNA BINDING MODES MAJOR ADDUCT OXALIPLATIN |
| ISSN号 | 0305-1048 |
| 通讯作者 | Wang, PY: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Structural properties of single DNA treated with anti-cancer drug cisplatin were studied with magnetic tweezers and AFM. Under the effect of low-concentration cisplatin, the DNA became more flexible, with the persistence length decreased significantly from 52 to 15 nm. At a high drug concentration, a DNA condensation phenomenon was observed. Based on experimental results from both single-molecule and AFM studies, we propose a model to explain this kind of DNA condensation by cisplatin: first, di-adducts induce local distortions of DNA. Next, micro-loops of 20 nm appear through distant crosslinks. Then, large aggregates are formed through further crosslinks. Finally, DNA is condensed into a compact globule. Experiments with Pt(dach)Cl(2) indicate that oxaliplatin may modify the DNA structures in the same way as cisplatin. The observed loop structure formation of DNA may be an important feature of the effect of platinum anti-cancer drugs that are analogous to cisplatin in structure. |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China; Innovation Project of the Chinese Academy of Sciences |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/34741]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hou, XM,Zhang, XH,Wei, KJ,et al. Cisplatin induces loop structures and condensation of single DNA molecules[J]. NUCLEIC ACIDS RESEARCH,2009,37(5):1400. |
| APA | Hou, XM.,Zhang, XH.,Wei, KJ.,Ji, C.,Dou, SX.,...&Wang, PY.(2009).Cisplatin induces loop structures and condensation of single DNA molecules.NUCLEIC ACIDS RESEARCH,37(5),1400. |
| MLA | Hou, XM,et al."Cisplatin induces loop structures and condensation of single DNA molecules".NUCLEIC ACIDS RESEARCH 37.5(2009):1400. |
入库方式： OAI收割
来源：物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。