Cloning, expression, and purification of soluble human interleukin-4 receptor in Streptomyces
文献类型：期刊论文
| 作者 | Zhang, Y ; Wang, WC ; Li, Y |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:1页码:139 |
| 关键词 | LIVIDANS PROTEINS BINDING ATOPY CELLS |
| ISSN号 | 1046-5928 |
| 通讯作者 | Zhang, Y: Chinese Acad Med Sci, Inst Med Biotechnol, Tiantan Xili 1, Beijing 100050, Peoples R China. |
| 中文摘要 | Interleukin-4 (IL-4) is a pleiotropic cytokine which plays a pivotal role in shaping immune responses and mediating important proinflammatory functions in asthma. This cytokine exerts its biological effects through binding to its receptor (IL-4R) complex, with the alpha chain as the high-affinity binding subunit. Soluble IL-4R (sIL-4R) lacks the transmembrane and cytoplasmic domains, so it cannot induce cellular activation. By acting as a decoy to circulating IL-4 and neutralizing its activity, the high specificity and affinity of sIL-4R make it ideal as an IL-4 antagonist. In this study, a sIL-4R cDNA encoding the extracellular domain of IL-4R alpha chain was cloned into a Streptomyces-Escherichia coli shuttle plasmid pSGLgpp and expressed secretly in Streptomyces lividans TK 24. On SDS-PAGE gel, the expressed sIL-4R protein showed a Mw of 24 kDa, agreeable with the predicted size. The N-terminal sequence of the protein was also determined, confirming its identity and indicating that no degradation occurred at the N-terminus. With DEAE-Sepharose Fast Flow and Superdex HR 75 columns, the protein was purified and used on HPLC analysis, the purity reaching about 90%. The results of the ligand-binding blot and ELISA showed that such protein has biological activity of binding with ligand IL-4. (C) 2004 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/34750]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Wang, WC,Li, Y. Cloning, expression, and purification of soluble human interleukin-4 receptor in Streptomyces[J]. PROTEIN EXPRESSION AND PURIFICATION,2004,36(1):139. |
| APA | Zhang, Y,Wang, WC,&Li, Y.(2004).Cloning, expression, and purification of soluble human interleukin-4 receptor in Streptomyces.PROTEIN EXPRESSION AND PURIFICATION,36(1),139. |
| MLA | Zhang, Y,et al."Cloning, expression, and purification of soluble human interleukin-4 receptor in Streptomyces".PROTEIN EXPRESSION AND PURIFICATION 36.1(2004):139. |
入库方式： OAI收割
来源：物理研究所
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