Comparison of phasing methods for sulfur-SAD using in-house chromium radiation: case studies for standard proteins and a 69 kDa protein
文献类型：期刊论文
| 作者 | Watanabe, N ; Kitago, Y ; Tanaka, I ; Wang, JW ; Gu, YX ; Zheng, CD ; Fan, HF |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
 |
| 出版日期 | 2005 |
| 卷号 | 61页码:1533 |
| 关键词 | SOFT X-RAYS DATA-COLLECTION WAVELENGTH ANOMALOUS SCATTERING DATA COMBINING DIRECT METHODS MACROMOLECULAR CRYSTALLOGRAPHY ROUTINE USE ISOMORPHOUS REPLACEMENT CRYSTAL-STRUCTURE DIFFRACTION RESOLUTION |
| ISSN号 | 0907-4449 |
| 通讯作者 | Watanabe, N: Hokkaido Univ, Grad Sch Sci, Sapporo, Hokkaido 0600810, Japan. |
| 中文摘要 | Phasing of the crystal structures of four standard proteins ( lysozyme, trypsin, glucose isomerase and thaumatin) and a novel 69 kDa protein from Thermus thermophilus, TT0570, was performed using the single-wavelength anomalous diffraction of S atoms intrinsically present within the native protein molecules. To utilize the sulfur anomalous diffraction, the data sets were collected using the loopless data-collection method with chromium K alpha X-rays of wavelength 2.29 alpha. Three phasing methods, MLPHARE, SHARP and OASIS-2004, were tested in combination with the DM or SOLOMON density-modification method. The results showed that the solvent contents are still an important factor for phasing with the S-SAD method, even when longer wavelength Cr K alpha radiation is used. Of the three procedures, the improved direct phasing of OASIS-2004 with its implemented fragment feedback to the direct-method probability calculation gave the best results in determining the initial phases. For all five proteins, almost the entire models could be built automatically. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/34960]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Watanabe, N,Kitago, Y,Tanaka, I,et al. Comparison of phasing methods for sulfur-SAD using in-house chromium radiation: case studies for standard proteins and a 69 kDa protein[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2005,61:1533. |
| APA | Watanabe, N.,Kitago, Y.,Tanaka, I.,Wang, JW.,Gu, YX.,...&Fan, HF.(2005).Comparison of phasing methods for sulfur-SAD using in-house chromium radiation: case studies for standard proteins and a 69 kDa protein.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,61,1533. |
| MLA | Watanabe, N,et al."Comparison of phasing methods for sulfur-SAD using in-house chromium radiation: case studies for standard proteins and a 69 kDa protein".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 61(2005):1533. |
入库方式： OAI收割
来源：物理研究所
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