Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant
文献类型：期刊论文
| 作者 | Cao, MW ; Deng, ML ; Wang, XL ; Wang, YL |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2008 |
| 卷号 | 112期号:43页码:13648 |
| 关键词 | DYNAMIC LIGHT-SCATTERING COIL-GLOBULE TRANSITION SELF-DIFFUSION NMR LONG DUPLEX DNA GENE DELIVERY COMPLEXES RELEASE TRANSFECTION COMPACTION MIXTURES |
| ISSN号 | 1520-6106 |
| 通讯作者 | Wang, YL: Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, Beijing 100190, Peoples R China. |
| 中文摘要 | Compaction of DNA by cationic gemini surfactant hexamethylene-1,6-bis-(dodecyldimethylammoniumbromide) (C12C6C12Br2) and the subsequent decompaction of the DNA-C12C6C12Br2 complexes by beta-cyclodextrin (beta-CD) or sodium dodecyl sulfate (SDS) have been studied by using potential and particle size measurements, atomic force microscopy (AFM), isothermal titration microcalorimetry (ITC), and circular dichroism. The results show that C12C6C12Br2 can induce the collapse of DNA into densely packed bead-like structures with smaller size in an all-or-none manner, accompanied by the increase of potential from highly negative values to highly positive values. In the decompaction of the DNA-C12C6C12Br2 complexes, beta-CD and SDS exhibit different behaviors. For beta-CD, the experimental results suggest that it can remove the outlayer hydrophobically bound C12C6C12Br2 Molecules from the DNA-C12C6C12Br2 complexes by inclusion interaction, and the excess beta-CD may attach on the complexes by forming inclusion complexes with the hydrocarbon chains of the electrostatically bound C12C6C12Br2 that cannot be removed. The increase of steric hindrance due to the attachment of beta-CD molecules results in the decompaction of the DNA condensates though the true release of DNA cannot be attained. However, for SDS, the experimental results suggest that it can realize the decompaction and release of DNA from its complexes with C12C6C12Br2 due to both ion-pairing and hydrophobic interaction between SDS and C12C6C12Br2. |
| 收录类别 | SCI |
| 资助信息 | National Science Foundation of China [20633010, 20573123] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/35657]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cao, MW,Deng, ML,Wang, XL,et al. Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2008,112(43):13648. |
| APA | Cao, MW,Deng, ML,Wang, XL,&Wang, YL.(2008).Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant.JOURNAL OF PHYSICAL CHEMISTRY B,112(43),13648. |
| MLA | Cao, MW,et al."Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant".JOURNAL OF PHYSICAL CHEMISTRY B 112.43(2008):13648. |
入库方式： OAI收割
来源：物理研究所
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