Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method
文献类型：期刊论文
| 作者 | Lu, H ; Wen, JA ; Wang, X ; Yuan, K ; Lu, HB ; Zhou, YL ; Jin, KJ ; Yang, GZ ; Li, W ; Ruan, KC |
| 刊名 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
 |
| 出版日期 | 2010 |
| 卷号 | 53期号:7页码:1230 |
| ISSN号 | 1674-7348 |
| 通讯作者 | Yang, GZ: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Mouse-Immunoglobulin G (mouse-IgG) with different concentrations in a range from 1000 to 0.0128 mu g/mL and a specific hybridization with goat anti-mouse IgG were detected successfully by using an oblique-incidence reflectivity difference (OI-RD) method. Two detection signals, consisting of an imaginary part (Im{Delta(p)-Delta(s)}) and a real part (Re{Delta(p)-Delta(s)}) of OI-RD, were obtained simultaneously. The detection results of hybridization by OI-RD were in accord with that of traditional fluorescent scans. In particular, we label-freely detected the washed mouse-IgG microarray with a series of concentrations and acquired a linear correlation between OI-RD intensities and the protein concentrations in logarithmic coordinates. The detection sensitivity of OI-RD can reach 14 fg. These experimental results suggest that the OI-RD method has potential applications in proteomics and clinical diagnosis. |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2007CB935700] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/35807]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lu, H,Wen, JA,Wang, X,et al. Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2010,53(7):1230. |
| APA | Lu, H.,Wen, JA.,Wang, X.,Yuan, K.,Lu, HB.,...&Ruan, KC.(2010).Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,53(7),1230. |
| MLA | Lu, H,et al."Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 53.7(2010):1230. |
入库方式： OAI收割
来源：物理研究所
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