Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method
文献类型：期刊论文
| 作者 | Lu, H ; Wen, JA ; Wang, X ; Yuan, K ; Li, W ; Lu, HB ; Zhou, YL ; Jin, KJ ; Ruan, KC ; Yang, GZ |
| 刊名 | JOURNAL OF OPTICS
 |
| 出版日期 | 2010 |
| 卷号 | 12期号:9 |
| 关键词 | PROTEOMICS |
| ISSN号 | 2040-8978 |
| 通讯作者 | Lu, H: Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | The specific binding between Cy5-labeled goat anti-mouse Immunoglobulin G (IgG) and mouse IgG with a concentration range from 625 to 10(4) mu g ml(-1) has been detected successfully by the oblique-incidence reflectivity difference (OI-RD) method in each procedure of microarray fabrication. The experimental data prove that the OI-RD method can be employed not only to distinguish the different concentrations in label-free fashion but also to detect the antibody-antigen capture. In addition, the differential treatment of the OI-RD signals can decrease the negative influences of glass slide as the microarray upholder. Therefore the OI-RD technique has promising applications for the label-free and high-throughput detection of protein microarrays. |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program in China [2007CB935700] |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/35813]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lu, H,Wen, JA,Wang, X,et al. Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method[J]. JOURNAL OF OPTICS,2010,12(9). |
| APA | Lu, H.,Wen, JA.,Wang, X.,Yuan, K.,Li, W.,...&Yang, GZ.(2010).Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method.JOURNAL OF OPTICS,12(9). |
| MLA | Lu, H,et al."Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method".JOURNAL OF OPTICS 12.9(2010). |
入库方式： OAI收割
来源：物理研究所
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