Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering
文献类型：期刊论文
| 作者 | Hong, XG ; Weng, YX ; Li, M |
| 刊名 | BIOPHYSICAL JOURNAL
 |
| 出版日期 | 2004 |
| 卷号 | 86期号:2页码:1082 |
| 关键词 | BIOLOGICAL MACROMOLECULES RHODOBACTER-SPHAEROIDES CRYSTAL-STRUCTURE ENERGY-TRANSFER B850 BAND SPECTROSCOPY RHODOPSIN DYNAMICS PROGRAM |
| ISSN号 | 0006-3495 |
| 通讯作者 | Weng, YX: Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, POB 603, Beijing 100080, Peoples R China. |
| 中文摘要 | The topological shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter spheroides 2.4.1 in detergent solution has been determined from synchrotron small-angle X-ray scattering data using direct curve-fitting by the ellipsoid, ab initio shape determination methods of simulated annealing algorithm and multipole expansion, respectively. The results indicate that the LH2 protein in aqueous solution is encapsulated by a monolayered detergent shell. The detergent-stabilized structure has the shape of an oblate plate, with a thickness of 40 Angstrom, a long axis of 110 Angstrom, and a short axis of 85 Angstrom. After correction for the detergent shell, the shape of the LH2 core is also an oblate plate with a height of 40 Angstrom, a long axis of 80 Angstrom, and a short axis of 55 Angstrom. In contrast to the cylindrical crystal structure with a height of 40 Angstrom and a diameter of 68 Angstrom, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ringlike crystal structure, with an eccentricity found to be 0.59-consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/35867]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hong, XG,Weng, YX,Li, M. Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering[J]. BIOPHYSICAL JOURNAL,2004,86(2):1082. |
| APA | Hong, XG,Weng, YX,&Li, M.(2004).Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering.BIOPHYSICAL JOURNAL,86(2),1082. |
| MLA | Hong, XG,et al."Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering".BIOPHYSICAL JOURNAL 86.2(2004):1082. |
入库方式： OAI收割
来源：物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。