DIRECT PHASING OF ONE-WAVELENGTH ANOMALOUS-SCATTERING DATA OF THE PROTEIN CORE STREPTAVIDIN
文献类型：期刊论文
| 作者 | BINGDONG, S ; SHENPING, L ; YUANXIN, G ; HAIFU, F ; KE, HM ; JIAXING, Y ; WOOLFSON, MM |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
 |
| 出版日期 | 1995 |
| 卷号 | 51页码:342 |
| 关键词 | COMBINING DIRECT METHODS ISOMORPHOUS REPLACEMENT MACROMOLECULAR CRYSTALLOGRAPHY CRYSTAL-STRUCTURE RESOLUTION |
| ISSN号 | 0907-4449 |
| 通讯作者 | BINGDONG, S: CHINESE ACAD SCI,INST PHYS,POB 603,BEIJING 100080,PEOPLES R CHINA. |
| 中文摘要 | The direct method [Fan, Hao, Gu, Qian, Zheng and Ke (1990). Acta Cryst. A46, 935-939] was used to break the phase ambiguity intrinsic to one-wavelength anomalous scattering data from a known protein of moderate size, core streptavidin, which was solved originally with three-wavelength anomalous diffraction data [Hendrickson, Pahler, Smith, Satow, Merritt and Phizackerley (1989). Proc. Natl Acad. Sci. USA, 86, 2190-2194]. Unlike that in the previous test with a small protein, the Fourier map calculated with the direct-method phases could not clearly reveal the moderate-sized protein structure. However, the phases can be improved step by step using Wang's solvent-flattening method, non-crystallographic symmetry averaging and the skeletonization method. The final electron-density map clearly shows most C-alpha positions and some side chains and it is traceable without prior knowledge of the structure. It is concluded that the direct method is capable of breaking the OAS phase ambiguity of a moderate-sized protein at moderate resolution such as 3 Angstrom, while the combination of direct methods with macromolecular techniques may produce phases good enough for unknown protein structure to be traced. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-09-17 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/36071]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | BINGDONG, S,SHENPING, L,YUANXIN, G,et al. DIRECT PHASING OF ONE-WAVELENGTH ANOMALOUS-SCATTERING DATA OF THE PROTEIN CORE STREPTAVIDIN[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,1995,51:342. |
| APA | BINGDONG, S.,SHENPING, L.,YUANXIN, G.,HAIFU, F.,KE, HM.,...&WOOLFSON, MM.(1995).DIRECT PHASING OF ONE-WAVELENGTH ANOMALOUS-SCATTERING DATA OF THE PROTEIN CORE STREPTAVIDIN.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,51,342. |
| MLA | BINGDONG, S,et al."DIRECT PHASING OF ONE-WAVELENGTH ANOMALOUS-SCATTERING DATA OF THE PROTEIN CORE STREPTAVIDIN".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 51(1995):342. |
入库方式： OAI收割
来源：物理研究所
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